{"title":"Species-Specific Sodium Iodide Symporters","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eThe sodium iodide symporter (NIS) is a membrane protein that drives uptake of iodine into cells. Mammals express NIS endogenously in the thyroid, salivary glands, gastric mucosa, and mammary glands. And other cell types and viruses can be engineered to express recombinant NIS.\u003c\/p\u003e\n\u003cp\u003eNIS concentrates radioactive iodine as well as several other radiotracers, so it can be used to noninvasively track cells \u003cem\u003ein vivo \u003c\/em\u003ethrough nuclear imaging. NIS imaging has several key advantages: it produces high-resolution 3D images that are fully quantitative, it is translational from small to large animals as well as humans, and it can be used for longitudinal imaging studies as the species-specific NIS proteins are non-immunogenic. \u003c\/p\u003e\n\u003ch3\u003eHow to use NIS for imaging\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eReagents:\u003c\/strong\u003e NIS concentrates a number of different radiotracers, several of which are clinically approved.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/NIS_radiotracers_large.jpg?3934735687258482264\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment:\u003c\/strong\u003e SPECT or PET machines can be used to detect radiotracer signal and biodistribution of NIS-expressing cells or virus. Combining SPECT or PET with computed tomography (CT) or magnetic resonance (MR) imaging gives the most informative results, as images can be co-registered to give anatomical localization of the radiotracer signal.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdditional considerations:\u003c\/strong\u003e Depending on the location of the target organs for imaging, administration of undiluted barium sulfate prior to imaging can be used to block endogenous NIS signal in the stomach.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWorkflow\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/NIS_workflow_1024x1024.jpg?5694603600312190400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eWhen to use NIS\u003c\/h3\u003e\n\u003cp\u003eNIS is well suited for longitudinal, noninvasive imaging studies in regenerative medicine, gene therapy, oncology, and oncolytic virotherapy, where it can be used to track the biodistribution of cell\/virus therapies or the destruction of tumors. NIS can be used for small or large animal imaging and is translatable to the clinic. The heart, lungs, liver, kidneys, orthopedics (joints and bone), and immune cells are all suitable organs for NIS imaging.\u003c\/p\u003e\n\u003cspan\u003eRadiotracers used for NIS imaging are actively pumped out of the brain and CNS. Therefore, unless the blood-brain barrier is permeated, NIS is not recommended for brain or CNS imaging. NIS imaging of organs near the stomach may be obscured by endogenous NIS signal from the stomach, which can be blocked with barium sulfate (see Additional considerations, above).\u003c\/span\u003e\u003cmeta charset=\"utf-8\"\u003e\n\u003ch3\u003e\u003c\/h3\u003e","products":[{"product_id":"lv008","title":"LV-mouseNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-mouse NIS lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in a variety of animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eMurine sodium iodide symporter (mNIS)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePublications that used associated LV-mNIS product:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Hickey et al. Noninvasive 3-dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene. \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25482651\" target=\"_blank\" title=\"NIS reporter gene for imaging hepatocyte transplantation engraftment\" rel=\"noopener noreferrer\"\u003e\u003cspan class=\"jrnl\" title=\"Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society\"\u003eLiver Transpl\u003c\/span\u003e. 2015\u003c\/a\u003e Apr;21(4):442-53.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV008_large.png?15362485046061481861\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-mNIS transduced HeLa H1 cells:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV008_large.png?18102218587092825979\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995962851400,"sku":"LV008-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15995963375688,"sku":"LV008-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV008.png?v=1467242960"},{"product_id":"lv020","title":"LV-eGFP-P2A-humanNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eLV-eGFP-P2A-hNIS purified high titer lentivirus stock\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: LV-SFFV-eGFP-P2A-hNIS. \u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the enhanced green fluorescent protein (eGFP) cDNA under control of the spleen focus-forming virus (SFFV) promoter linked to the human sodium iodide symporter (hNIS) cDNA via a P2A cleavage peptide (see below). The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR (Miyoshi et al. J Virol. 1998. 72:8150-8157).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter\u003c\/strong\u003e: 1e8 TU\/ml in DMEM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSize\u003c\/strong\u003e: 0.25 mL or 1.0 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human and rodent cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Enhanced GFP linked to human NIS by a P2A sequence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Lv020_construct_large.png?v=1478465822\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFluorescence Expression: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-eGFP-P2A-hNIS at the indicated MOIs. After 6 days fluorescence expression of the transduced (green) and untransducted parental HeLaH1 cells (grey) was analyzed by flow cytometry.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV020_Flow_medium.png?v=1478466257\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNIS Gene Expression: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-eGFP-P2A-hNIS at the indicated MOIs. After 1 week \u003csup\u003e125\u003c\/sup\u003eI uptake by the cells was measured in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV020_Uptake_NIS_medium.png?v=1478466759\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996154445896,"sku":"LV020-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996154773576,"sku":"LV020-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Lv020_construct.png?v=1478465822"},{"product_id":"lv001","title":"LV-humanNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-human NIS lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eHuman sodium iodide symporter (hNIS)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eVector Map:\u003cimg alt=\"Human sodium iodide symporter (NIS) lentivirus construct\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV001_large.png?18188218167522186448\" style=\"float: none;\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-hNIS transduced HeLa H1 cells:\u003cbr\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV001_large.png?6569600138550727453\" alt=\"\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995932115016,"sku":"LV001-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15995933229128,"sku":"LV001-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV001.png?v=1467242935"},{"product_id":"lv023","title":"LV-Luc2-P2A-humanNIS","description":"\u003cp\u003e\u003cb\u003e\u003cspan style=\"color: #0000ff;\"\u003eLV-Luc2-P2A-human NIS\u003c\/span\u003e purified high titer lentivirus stock\u003c\/b\u003e\u003c\/p\u003e\n\u003cul _mce_style=\"font-size: 14px; font-weight: normal;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: LV-SFFV-Luc2-P2A-hNIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter\u003c\/strong\u003e: 1e8 TU\/ml in DMEM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSize\u003c\/strong\u003e: 0.25 mL or 1.0 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human and rodent cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Firefly luciferase (Luc2) linked to human NIS (hNIS) by a P2A sequence.\u003c\/li\u003e\n\u003cli\u003eEnvelope: VSV.G pseudotyped HIV-1 based lentiviral vector\u003c\/li\u003e\n\u003cli\u003eTropism: Broad range of species\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul _mce_style=\"font-size: 14px; font-weight: normal;\"\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: In vitro for transduction of primary cells and cell lines.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996157526088,"sku":"LV023-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996157657160,"sku":"LV023-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV023.png?v=1467242959"},{"product_id":"lv019","title":"LV-humanNIS-P2A-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-human NIS-P2A-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eHuman sodium iodide symporter (hNIS) linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV019_large.png?11907679721359718223\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-hNIS-P2A-Puro transduced HeLa H1 cells:\u003cbr\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV019_large.jpg?10188034229019917655\" alt=\"\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996151005256,"sku":"LV019-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996153921608,"sku":"LV019-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV019.png?v=1467242949"},{"product_id":"lv007","title":"LV-ratNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-rat NIS stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes:\u003c\/strong\u003e Rat sodium iodide symporter (rNIS)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV007_large.png?17835535312393857495\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-rNIS transduced HeLa H1 cells:\u003cbr\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV007_large.jpg?17722758243467155298\" alt=\"\" width=\"389\" height=\"236\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995961704520,"sku":"LV007-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15995962458184,"sku":"LV007-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV007.png?v=1467242978"},{"product_id":"lv004","title":"LV-dogNIS","description":"\u003cp\u003e\u003cb\u003e\u003cspan style=\"color: #0000ff;\"\u003eLV-dNIS\u003c\/span\u003e purified high titer stock\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: LV-SFFV-dog NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter\u003c\/strong\u003e: 1e8 TU\/ml in DMEM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSize: \u003c\/strong\u003e0.25 mL or 1.0 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Dog sodium iodide symporter (dNIS)\u003c\/li\u003e\n\u003cli\u003eEnvelope: VSV.G pseudotyped HIV-1 based lentiviral vector\u003c\/li\u003e\n\u003cli\u003eTropism: Broad range of species\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: In vitro for transduction of primary cells and cell lines.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995936735304,"sku":"LV004-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15995938406472,"sku":"LV004-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV004.png?v=1467242896"},{"product_id":"lv014","title":"LV-dogNIS-PGK-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-dog NIS-PGK-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoters:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models. Mouse phosphoglycerate kinase I (PGK) promoter drives puromycin resistance for selection of high dNIS-expressing transduced cells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eCanine sodium iodide symporter (dNIS) and the puromycin resistance gene (Puro).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV014_large.png?6197614129715181261\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-dNIS-PGK-Puro transduced HeLa H1 cells:\u003cbr\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV014_large.jpg?10841819570254790126\" alt=\"\" width=\"431\" height=\"262\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995974844488,"sku":"LV014-S-STAN","price":245.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV014.png?v=1467242905"},{"product_id":"lv017","title":"LV-rhesusNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-Rhesus NIS stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eRhesus sodium iodide symporter (RhNIS)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"line-height: 1.4;\"\u003eEnvelope\/Tropism: \u003c\/strong\u003e\u003cspan style=\"line-height: 1.4;\"\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV017_large.png?1030075202480104966\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-RhNIS transduced HeLa H1 cells:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV017_large.jpg?722573253620317856\" alt=\"\" width=\"392\" height=\"240\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995976908872,"sku":"LV017-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15995977072712,"sku":"LV017-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV017.png?v=1467242986"},{"product_id":"lv013","title":"LV-humanNIS-IRES-Neo","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-human NIS-IRES-Neo lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eHuman sodium iodide symporter (hNIS) linked to the neomycin resistance gene (Neo) via an internal ribosomal entry site (IRES).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV013_large.png?409228336412606599\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-hNIS-IRES-Neo transduced HeLa H1 cells:\u003cbr\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV013_large.jpg?2525247234461769647\" alt=\"\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15995972517960,"sku":"LV013-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15995972780104,"sku":"LV013-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV013.png?v=1467242940"},{"product_id":"dna1001","title":"pLV-SFFV-humanNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-human NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-SFFV-hNIS plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Human sodium iodide symporter (hNIS)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1001_map_d9efc2d7-ed2d-4645-add8-59c04a087d8b_large.png?v=1488818927\" alt=\"lentivector, plasmid map, hNIS, NIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003e\u003cspan\u003eUptake assay of LV-hNIS transduced cells in the presence or absence of KClO\u003c\/span\u003e\u003csub\u003e4\u003c\/sub\u003e\u003cspan\u003e, an inhibitor of NIS-mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI  uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1001_uptake_large.jpg?v=1488818283\" alt=\"Uptake, hNIS, NIS\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":1079649235,"sku":"(DNA1001-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1011","title":"pLV-SFFV-ratNIS","description":"\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-rat NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-SFFV-rNIS plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Rat NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding rat NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1011_map_large.jpg?v=1488820029\" alt=\"lentivector, plasmid map, rNIS, NIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eUptake assay of LV-rNIS transduced cells in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI  uptake.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1011_uptake_d0518085-b08b-46b3-8695-6912366ccbbf_large.png?v=1488820409\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":1128750043,"sku":"(DNA1011-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1042","title":"pLV-SFFV-mouseNIS-PGK-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-mouse NIS-PGK-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-mNIS-PGK-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. \u003cspan\u003ePGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Mouse sodium iodide symporter (mNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding mouse NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1042_map_large.png?v=1489165338\" alt=\"plasmid map mNIS puro\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003e\u003cspan\u003eHeLaH1 cells were transduced with\u003c\/span\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cspan\u003eLV-mNIS-PGK-Puro and selected with puromycin. \u003c\/span\u003e\u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1042_uptake_large.png?v=1489165555\" alt=\"Uptake, mNIS, NIS\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":1128769667,"sku":"(DNA1042-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1018","title":"pLV-SFFV-ratNIS-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-rat NIS-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-rNIS-IRES-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Rat sodium iodide sumporter (rNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker:\u003c\/strong\u003e IRES\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding rat NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1018_map_8860f03a-63c6-4125-adbe-dfcda1acf5c1_large.jpg?v=1488903394\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-rNIS-Puro and selected with puromycin. \u003cspan\u003eNIS mediated \u003csup\u003e125\u003c\/sup\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1018_uptake_large.png?v=1488903422\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":1128771047,"sku":"(DNA1018-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"lv022","title":"LV-mouseNIS-PGK-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-murine NIS-PGK-Puro stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models. Mouse phosphoglycerate kinase I (PGK) promoter drives puromycin resistance for selection of high mNIS-expressing transduced cells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eMurine sodium iodide symporter (mNIS) and the puromycin resistance gene (Puro).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"line-height: 1.4;\"\u003eEnvelope\/Tropism: \u003c\/strong\u003e\u003cspan style=\"line-height: 1.4;\"\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePublications that used associated LV-mNIS product:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Hickey et al. Noninvasive 3-dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene. \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25482651\" target=\"_blank\" title=\"NIS reporter gene for imaging hepatocyte transplantation engraftment\" rel=\"noopener noreferrer\"\u003e\u003cspan class=\"jrnl\" title=\"Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society\"\u003eLiver Transpl\u003c\/span\u003e. 2015\u003c\/a\u003e Apr;21(4):442-53.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV022_large.png?9694022372690777348\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-mNIS-PGK-Puro transduced HeLa H1 cells:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV022_large.jpg?2713291023473022095\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996156936264,"sku":"LV022-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996157067336,"sku":"LV022-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV022.png?v=1467242974"},{"product_id":"dna1040","title":"pLV-SFFV-eGFP-P2A-humanNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-eGFP-P2A-human NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-eGFP-P2A-hNIS plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells,\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Enhanced green fluorescent protein (eGFP) and human sodium iodide symporter (hNIS)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eSelf-cleavage peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding enhanced GFP and human NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1040_map_large.jpg?v=1489161428\" alt=\"plasmid map, eGFP hNIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFlow cytometry: \u003c\/strong\u003eLV-eGFP-hNIS transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1040_flow_large.jpg?v=1489161504\" alt=\"flow cytometry eGFP\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUptake Assay: \u003c\/strong\u003eNIS mediated \u003csup\u003e125\u003c\/sup\u003eI uptake of LV-eGFP-hNIS transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1040_uptake_large.jpg?v=1489161546\" alt=\"uptake\"\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":9148129091,"sku":"(DNA1040-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1043","title":"pLV-SFFV-Luc2-P2A-humanNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-Luc2-P2A-human NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-Luc2-P2A-hNIS plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Human sodium iodide symporter (hNIS) and firefly luciferase (Luc2)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eSelf-cleaving peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding firefly luciferase and human NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1043_map_large.png?v=1489166243\" alt=\"plasmid map luc2 hNIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eTransgene Validation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLuciferase Assay: \u003c\/strong\u003e\u003cspan\u003e10\u003c\/span\u003e\u003csup\u003e4\u003c\/sup\u003e\u003cspan\u003e, 10\u003c\/span\u003e\u003csup\u003e5\u003c\/sup\u003e\u003cspan\u003e, or 10\u003c\/span\u003e\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e LV-Luc2-hNIS transduced cells were placed in wells of a 96-well plate and 0.3 mg of d-luciferin was added to the indicated wells. The plate was immediately imaged using a Xenogen IVIS Spectrum.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1043_luc_large.jpg?v=1489166290\" alt=\"luciferase IVIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUptake Assay: \u003c\/strong\u003e\u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake of \u003c\/span\u003e\u003cspan\u003eLV-Luc2-hNIS transduced cells \u003c\/span\u003e\u003cspan\u003ewas assayed in the presence or absence of KClO\u003c\/span\u003e\u003csub\u003e4\u003c\/sub\u003e\u003cspan\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1043_uptake_large.png?v=1489166316\" alt=\"Uptake, hNIS, NIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":9148179651,"sku":"(DNA1043-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1044","title":"pLV-SFFV-iRFP-P2A-humanNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-iRFP-P2A-human NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-iRFP-P2A-hNIS plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells,\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e:  Near-infrared fluorescent protein (iRFP; excitation: 690nm, emission: 713nm) and human sodium iodide symporter (hNIS)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eSelf-cleavage peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding near-infrared fluorescent protein and human NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1044_map_large.jpg?v=1489412638\" alt=\"plasmid map iRFP hNIS\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFlow Cytometry:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eLV-iRFP-hNIS transduced (red) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1044_flow_43bc8867-e80d-4565-b62a-2a5fde52886a_large.png?v=1489412853\" alt=\"iRFP flow cytometry\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eUptake Assay: \u003c\/strong\u003eNIS mediated \u003csup\u003e125\u003c\/sup\u003eI uptake of LV-iRFP-hNIS transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1044_uptake_large.png?v=1489412898\" alt=\"Uptake, hNIS, NIS\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":9148225603,"sku":"(DNA1044-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1041","title":"pLV-SFFV-humanNIS-P2A-eGFP-T2A-Luc2","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-human NIS-P2A-eGFP-T2A-Luc2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-hNIS-P2A-eGFP-T2A-Luc2 plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells,\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Human sodium iodide symporter (hNIS), enhanced green fluorescent protein (eGFP), and firefly luciferase (Luc2)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinkers: \u003c\/strong\u003eSelf-cleavage peptides P2A and T2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human NIS, enhanced GFP, and firefly luciferase in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1041_map_large.png?v=1489163994\" alt=\"plasmid map hNIS eGFP Luc2\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUptake Assay: \u003c\/strong\u003eNIS mediated \u003csup\u003e125\u003c\/sup\u003eI uptake of \u003cspan\u003eLV-hNIS-eGFP-Luc2 transduced cells \u003c\/span\u003ewas assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1041_uptake_large.png?v=1489164164\" alt=\"Uptake, hNIS, NIS\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFlow Cytometry:\u003c\/strong\u003e \u003cspan\u003eLV-hNIS-eGFP-Luc2 transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1041_flow_large.jpg?v=1489164238\" alt=\"flow cytometry eGFP\" width=\"411\" height=\"395\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eLuciferase Assay:\u003c\/strong\u003e 10\u003csup\u003e4\u003c\/sup\u003e, 10\u003csup\u003e5\u003c\/sup\u003e, or 10\u003csup\u003e6\u003c\/sup\u003e LV-hNIS-eGFP-Luc2 transduced cells were placed in wells of a 96-well plate and 0.3 mg of d-luciferin was added to the indicated wells. The plate was immediately imaged using a Xenogen IVIS Spectrum.\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1041_luc_large.jpg?v=1489164684\" alt=\"luciferase assay\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":9149225667,"sku":"(DNA1041-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"lv024","title":"LV-iRFP-P2A-humanNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-iRFP-P2A-human NIS lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eNear-infrared fluorescent protein (iRFP) linked to the human sodium iodide symporter (hNIS) via a P2A cleavage peptide. iRFP has excitation and emission of 690 and 713 nm, respectively. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV024_large.png?5087696611348034344\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFlow cytometry of LV-iRFP-P2A-hNIS transduced HeLa H1 cells:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Flow_for_website_LV024_large.jpg?5335967432164592108\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-iRFP-P2A-hNIS transduced HeLa H1 cells:\u003cbr\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV024_large.jpg?3531912333177700030\" alt=\"\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25mL","offer_id":15996157984840,"sku":"LV024-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0mL","offer_id":15996158443592,"sku":"LV024-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV024.png?v=1467242951"},{"product_id":"lv025","title":"LV-mouseNIS-P2A-Neo","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-murine NIS-P2A-Neo lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in a variety of animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eMurine sodium iodide symporter (mNIS) linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"line-height: 1.4;\"\u003eEnvelope\/Tropism: \u003c\/strong\u003e\u003cspan style=\"line-height: 1.4;\"\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePublications that used associated LV-mNIS product:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Hickey et al. Noninvasive 3-dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene. \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25482651\" target=\"_blank\" title=\"NIS reporter gene for imaging hepatocyte transplantation engraftment\" rel=\"noopener noreferrer\"\u003e\u003cspan class=\"jrnl\" title=\"Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society\"\u003eLiver Transpl\u003c\/span\u003e. 2015\u003c\/a\u003e Apr;21(4):442-53.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV025_large.png?9826028778311775769\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-mNIS-P2A-Neo transduced HeLa H1 cells:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_webside_LV025_large.jpg?12243113113326719633\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996160311368,"sku":"LV025-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996160344136,"sku":"LV025-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV025.png?v=1467242963"},{"product_id":"lv026","title":"LV-ratNIS-IRES-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-rat NIS-IRES-Puro stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eRat sodium iodide symporter (rNIS) linked to the puromycin resistance gene (Puro) via an internal ribosomal entry site (IRES).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"line-height: 1.4;\"\u003eEnvelope\/Tropism: \u003c\/strong\u003e\u003cspan style=\"line-height: 1.4;\"\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Schematic_for_website_LV026_large.png?4089165318753059804\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (\u003csup\u003e125\u003c\/sup\u003eI) Uptake Assay of LV-rNIS-IRES-Puro transduced HeLa H1 cells:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_for_website_LV026_large.jpg?6042037592588634721\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996163981384,"sku":"LV026-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996164309064,"sku":"LV026-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Schematic_for_website_LV026.png?v=1467243047"},{"product_id":"lv021","title":"LV-humanNIS-P2A-eGFP-T2A-Luc2","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-hNIS-P2A-eGFP-T2A-Luc2 lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReporter genes:\u003c\/strong\u003e Human sodium iodide symporter (hNIS), Enhanced green fluorescent protein (eGFP), Firefly luciferase (Luc2).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the human sodium iodide symporter (hNIS) cDNA under control of the spleen focus-forming virus (SFFV) promoter linked to the enhanced green fluorescent protein (eGFP) cDNA via a P2A cleavage peptide and the firefly luciferase (Luc2) cDNA via a T2A cleavage peptide (see below). This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 5e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV021_construct_large.png?v=1478378903\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eIodine (I-125) Uptake Assay of LV-hNIS-P2A-eGFP-T2A-Luc2 transduced HeLa H1 cells: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-hNIS-P2A-eGFP-T2A-Luc2 at the indicated MOIs. After 1 week I-125 uptake by the cells was measured in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated I-125 uptake.\u003cstrong\u003e\u003cbr\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Uptake_for_COA_LV-IM27_large.jpg?v=1478378834\" style=\"float: none;\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFluorescence Expression: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-hNIS-P2A-eGFP-T2A-Luc2 at the indicated MOIs. After 6 days fluorescence expression of the transduced (green) and untransducted parental HeLaH1 cells (grey) was analyzed by flow cytometry.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Flow_for_COA_LV-IM27_large.jpg?v=1478378981\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLuciferase Expression\u003c\/strong\u003e\u003cstrong\u003e: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-hNIS-P2A-eGFP-T2A-Luc2 at the indicated MOIs. To measure luciferase expression, 10\u003csup\u003e4\u003c\/sup\u003e, 10\u003csup\u003e5\u003c\/sup\u003e, or 10\u003csup\u003e6\u003c\/sup\u003e cells were seeded in wells of a 96-well plate and 3 mg\/mL of d-luciferin was added to the indicated wells. The plate was immediately imaged using the IVIS Spectrum imaging system.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV021_Luc_large.png?v=1478379173\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996155756616,"sku":"LV021-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996155985992,"sku":"LV021-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Uptake_for_COA_LV-IM27.jpg?v=1478378834"},{"product_id":"lv036","title":"LV-PGK-humanNIS-P2A-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-PGK-hNIS-P2A-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Phosphoglycerate kinase (PGK) promoter for constitutive gene expression in numerous cell types. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReporter gene:\u003c\/strong\u003e Human sodium iodide symporter (hNIS)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelection gene:\u003c\/strong\u003e Puromycin (Puro)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the human sodium iodide symporter (hNIS) cDNA under control of the phosphoglycerate kinase (PGK) promoter linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide (see below). This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV036_construct_large.png?v=1478379787\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eIodine (I-125) Uptake Assay of transduced HeLa H1 cells: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-PGK-hNIS-P2A-Puro (MOI = 5) and after 3 days the cells were amplified under puromycin selection. Uptake of \u003csup\u003e125\u003c\/sup\u003eI by 3 x 10\u003csup\u003e5\u003c\/sup\u003e transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/Uptake_for_COA_LV-IM26_large.jpg?v=1478379719\" style=\"float: none;\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996329001032,"sku":"LV036-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996329328712,"sku":"LV036-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV036_construct.png?v=1478750082"},{"product_id":"lv037","title":"LV-EF1α-humanNIS-P2A-Puro","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-EF1a-hNIS-P2A-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Elongation factor-1 alpha (EF1a)\u003cspan\u003e \u003c\/span\u003efor constitutive gene expression in numerous cell types. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReporter gene:\u003c\/strong\u003e Human sodium iodide symporter (hNIS) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelection gene\u003c\/strong\u003e: Puromycin (Puro)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the human sodium iodide symporter (hNIS) cDNA under control of the EF1a promoter linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide (see below). This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV037_Construct_large.png?v=1478467870\" style=\"float: none;\" width=\"452\" height=\"111\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (I-125) Uptake Assay of transduced HeLa H1 cells: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-EF1a-hNIS-P2A-Puro (MOI = 5) and after 3 days the cells were amplified under puromycin selection. Uptake of \u003csup\u003e125\u003c\/sup\u003eI by 3 x 10\u003csup\u003e5\u003c\/sup\u003e transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV037_Uptake_NIS_large.png?v=1478467964\" style=\"float: none;\" width=\"344\" height=\"260\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996329984072,"sku":"LV037-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996331622472,"sku":"LV037-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV037_Construct.png?v=1478467870"},{"product_id":"lv040","title":"LV-EF1α-rhesusNIS-PGK-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-EF1a-Rhesus NIS-PGK-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoters:\u003c\/strong\u003e Elongation factor-1 alpha (EF1a) and phosphoglycerate kinase (PGK)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eRhesus sodium iodide symporter (RhNIS) driven by the EF1a promoter and puromycin (Puro) antibiotic resistance gene driven by the PGK promoter\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003e\u003cspan\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the rhesus sodium iodide symporter (RhNIS) cDNA under control of the elongation factor-1 alpha (EF1α) promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter (see below). The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV040_construct_large.png?v=1478715769\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNIS Expression: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-EF1α-RhNIS-PGK-Puro (MOI = 10). Uptake of \u003csup\u003e125\u003c\/sup\u003eI by 3 x 10\u003csup\u003e5\u003c\/sup\u003e transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV040_Uptake_large.png?v=1478715771\" style=\"float: none;\" width=\"332\" height=\"246\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996335226952,"sku":"LV040-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996335390792,"sku":"LV040-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV040_construct.png?v=1478715769"},{"product_id":"lv018","title":"LV-rhesusNIS-PGK-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cstrong\u003eHigh-titer purified LV-Rhesus NIS-PGK-Puro lentivirus stocks\u003c\/strong\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoters:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) for high constitutive NIS gene expression and phosphoglycerate kinase (PGK) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eRhesus sodium iodide symporter (RhNIS) driven by the SFFV promoter and puromycin (Puro) antibiotic resistance gene driven by the PGK promoter\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003e\u003cspan\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the rhesus sodium iodide symporter (RhNIS) cDNA under control of the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter (see below). The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV018_construct_large.png?v=1478734506\" style=\"float: none;\" width=\"443\" height=\"83\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNIS Expression:\u003c\/strong\u003e HeLaH1 cells were transduced with LV-RhNIS-PGK-Puro (MOI = 40). Uptake of \u003csup\u003e125\u003c\/sup\u003eI by 2 x 10\u003csup\u003e5\u003c\/sup\u003e transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV018_Uptake_large.png?v=1478734508\" style=\"float: none;\" width=\"338\" height=\"270\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996150251592,"sku":"LV018-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996150120520,"sku":"LV018-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV018_construct.png?v=1478734506"},{"product_id":"lv042","title":"LV-CAG-humanNIS-P2A-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-CAG-hNIS-P2A-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Synthetic hybrid CAG promoter (\u003cspan\u003eCMV enhancer, chicken beta-Actin \u003c\/span\u003epromoter\u003cspan\u003e and rabbit beta-Globin splice acceptor site) \u003c\/span\u003efor constitutively high levels of gene expression in numerous cell types. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReporter gene:\u003c\/strong\u003e Human sodium iodide symporter (hNIS) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelection gene\u003c\/strong\u003e: Puromycin (Puro)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the human sodium iodide symporter (hNIS) cDNA under control of the CAG promoter linked to the puromycin resistance gene (Neo) via a P2A cleavage peptide (see below). This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV042_construct_large.png?v=1478736895\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eIodine (I-125) Uptake Assay of transduced HeLa H1 cells: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-CAG-hNIS-P2A-Puro (MOI = 5) and after 3 days the cells were amplified under puromycin selection. Uptake of \u003csup\u003e125\u003c\/sup\u003eI by 3 x 10\u003csup\u003e5\u003c\/sup\u003e transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV042_uptake_large.png?v=1478736897\" style=\"float: none;\" width=\"313\" height=\"253\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996336439368,"sku":"LV042-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996336996424,"sku":"LV042-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV042_construct.png?v=1478736895"},{"product_id":"lv038","title":"LV-Ub-humanNIS-P2A-Puro","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-Ub-hNIS-P2A-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter:\u003c\/strong\u003e Ubiquitin (Ub) promoter for constitutive gene expression in numerous cell types. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReporter gene:\u003c\/strong\u003e Human sodium iodide symporter (hNIS) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelection gene\u003c\/strong\u003e: Puromycin (Puro)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the human sodium iodide symporter (hNIS) cDNA under control of the Ubiquitin (Ub) promoter linked to the puromycin resistance gene (Neo) via a P2A cleavage peptide (see below). This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV038_construct_large.png?v=1478749885\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIodine (I-125) Uptake Assay of transduced HeLa H1 cells: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-Ub-hNIS-P2A-Puro (MOI = 5) and after 3 days the cells were amplified under puromycin selection. Uptake of \u003csup\u003e125\u003c\/sup\u003eI by 3 x 10\u003csup\u003e5\u003c\/sup\u003e transduced cells was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated \u003csup\u003e125\u003c\/sup\u003eI uptake.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV038_Uptake_large.png?v=1478749886\" style=\"float: none;\" width=\"368\" height=\"277\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996332802120,"sku":"LV038-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996333064264,"sku":"LV038-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV038_construct.png?v=1478749885"},{"product_id":"lv049","title":"LV-humanNISplus-PGK-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-hNISplus-PGK-Puro lentivirus stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoter: \u003c\/strong\u003eSpleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models. Phosphoglycerate kinase promoter for lower level constitutive expression in numerous cell types.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReporter gene:\u003c\/strong\u003e New human sodium iodide symporter (hNIS) with enhanced uptake of the PET tracer, F18-tetrafluoroborate \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelection gene\u003c\/strong\u003e: Puromycin (Puro)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the human NIS genetically modified for enhanced uptake for tetrafluoroborate (NISplus). The hNISplus cDNA is under control of the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter (see below). The hNIS also encodes a HA tag which allows easy detection of hNIS expression using anti-HA antibodies. The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 0.5-2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e Vector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV040_schematic_large.png?v=1479305048\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eNIS Expression:\u003c\/strong\u003e The functionality of the hNIS gene was assessed using an uptake assay; uptake of F18-tetrafluoroborate (F18-TFB) by lentivirus-transduced HeLaH1 cells in 96-well plates was measured in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of NIS-mediated F18-TFB uptake. Optimized hNISplus increases uptake by 30% as compared to WT hNIS.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV049_uptake_large.png?v=1479305637\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"padding-left: 30px;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL","offer_id":31401720131,"sku":"LV049-S","price":225.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":31401720195,"sku":"LV049-L","price":475.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV040_schematic.png?v=1479305630"},{"product_id":"dna1006","title":"pLV-SFFV-humanNIS-Neo","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-human NIS-Neo\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-hNIS-IRES-Neo plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Human sodium iodide symporter (hNIS) and neomycin resistance (Neo)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eIRES\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human NIS and neomycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Map_1006_5951ad4b-6c50-47ba-8392-7940c933bfe1_large.png?v=1488561279\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were trasduced with LV-hNIS-Neo and selected with G418. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"Uptake, hNIS, NIS\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Uptake_1006_large.jpg?v=1488561183\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":34939230659,"sku":"(DNA1006-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1010","title":"pLV-SFFV-mouseNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-mouse NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-SFFV-mNIS plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Mouse NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding mouse NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1010_map2_large.png?v=1488819660\" alt=\"mNIS, lentivector, plasmid, NIS\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003e\u003cspan\u003eUptake assay of LV-mNIS transduced cells in the presence or absence of KClO\u003c\/span\u003e\u003csub\u003e4\u003c\/sub\u003e\u003cspan\u003e, an inhibitor of NIS-mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI  uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1010_map_large.png?v=1488819704\" alt=\"Uptake, mNIS, NIS\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35011924675,"sku":"(DNA1010-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1019","title":"pLV-SFFV-HA-humanNIS-PGK-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-HA-human NIS-PGK-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-HA-hNIS-PGK-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. \u003cspan\u003ePGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: HA-tagged human sodium iodide symporter (HA-hNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEpitope tag:\u003c\/strong\u003e \u003cspan\u003eHuman influenza \u003c\/span\u003ehemagglutinin tag\u003cem\u003e (\u003c\/em\u003eHA)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human sodium iodide symporter and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1019_map_large.jpg?v=1488904707\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-HA-hNIS-PGK-Puro and selected with puromycin. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1019_uptake_large.jpg?v=1488904737\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35039829443,"sku":"(DNA1019-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1035","title":"pLV-SFFV-dogNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-dNIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-SFFV-dog NIS plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Dog sodium iodide symporter (dNIS)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding dog NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1035_map_large.jpg?v=1488989498\" alt=\"plasmid map, dog NIS\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003e\u003cspan\u003eUptake assay of LV-dNIS transduced cells in the presence or absence of KClO\u003c\/span\u003e\u003csub\u003e4\u003c\/sub\u003e\u003cspan\u003e, an inhibitor of NIS-mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI  uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1035_uptake_large.jpg?v=1488989608\" alt=\"Uptake, dNIS, NIS\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35062510531,"sku":"(DNA1035-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1037","title":"pLV-SFFV-rhesusNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-RhNIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-SFFV-Rhesus NIS plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Rhesus sodium iodide symporter (RhNIS)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding Rhesus NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1037_map_large.jpg?v=1488992103\" alt=\"plasmid map, RhNIS\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003e\u003cspan\u003eUptake assay of LV-RhNIS transduced cells in the presence or absence of KClO\u003c\/span\u003e\u003csub\u003e4\u003c\/sub\u003e\u003cspan\u003e, an inhibitor of NIS-mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI  uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1037_uptake_large.png?v=1488992145\" alt=\"uptake, RhNIS\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35063786627,"sku":"(DNA1037-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1045","title":"pLV-SFFV-mouseNIS-P2A-Neo","description":"\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-mouse NIS-P2A-Neo\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-SFFV-mNIS-P2A-Neo plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Mouse sodium iodide symporter (mNIS) and neomycin resistance (Neo)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker:\u003c\/strong\u003e Self-cleaving peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding mouse NIS and neomycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1045_map_large.png?v=1489413391\" alt=\"plasmid map mNIS Neo\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-mNIS-Neo and selected with G418. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1045_uptake_large.png?v=1489413431\" alt=\"Uptake, mNIS, NIS\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35217818307,"sku":"(DNA1045-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1058","title":"pLV-PGK-humanNIS-P2A-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-PGK-human NIS-P2A-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-PGK-hNIS-P2A-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: Phosphoglycerate kinase I promoter (PGK) \u003cspan\u003efor constitutive gene expression in numerous cell types. \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Human sodium iodide symporter (hNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eSelf-cleavage peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1058_map_large.png?v=1489501583\" alt=\"plasmid map PGK hNIS puro\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHelaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-PGK-hNIS-P2A-Puro  and selected with puromycin. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1058_uptake_large.jpg?v=1489501556\" alt=\"uptake pgk hnis puro\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35257281923,"sku":"(DNA1058-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1059","title":"pLV-PGK-mouseNIS-P2A-Neo","description":"\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-PGK-mouse NIS-P2A-Neo\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-PGK-mNIS-P2A-Neo plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: \u003cspan\u003e Phosphoglycerate kinase I (PGK) promoter for constitutive gene expression in numerous cell types. \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Mouse sodium iodide symporter (mNIS) and neomycin resistance (Neo)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker:\u003c\/strong\u003e Self-cleaving peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding mouse NIS and neomycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1059_map_large.png?v=1489502882\" alt=\"plasmid map pgk mnis puro\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-PGK-mNIS-Neo and selected with G418. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1059_uptake_large.png?v=1489502928\" alt=\"uptake pgk mnis puro\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35257712259,"sku":"(DNA1059-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1060","title":"pLV-EF1a-humanNIS-P2A-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-EF1a-human NIS-P2A-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-EF1a-hNIS-P2A-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: \u003cspan\u003eElongation factor-1 alpha (EF1a)\u003c\/span\u003e\u003cspan\u003e promoter \u003c\/span\u003e\u003cspan\u003efor constitutive gene expression in numerous cell types, particularly in cells where other promoters are silenced.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Human sodium iodide symporter (hNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eSelf-cleavage peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1060_map_large.png?v=1489505676\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHelaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-EF1a-hNIS-P2A-Puro  and selected with puromycin. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1060_uptake_large.jpg?v=1489505708\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35258518211,"sku":"(DNA1060-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1063","title":"pLV-CAG-mouseNIS-P2A-Neo","description":"\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-CAG-mouse NIS-P2A-Neo\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-CAG-mNIS-P2A-Neo plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: \u003cspan\u003eSynthetic hybrid CAG promoter (\u003c\/span\u003e\u003cspan\u003eCMV enhancer, chicken beta-Actin \u003c\/span\u003e\u003cspan\u003epromoter\u003c\/span\u003e\u003cspan\u003e and rabbit beta-Globin splice acceptor site) \u003c\/span\u003e\u003cspan\u003efor constitutively high levels of gene expression in numerous cell types.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Mouse sodium iodide symporter (mNIS) and neomycin resistance (Neo)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker:\u003c\/strong\u003e Self-cleaving peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding mouse NIS and neomycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1063_map_large.png?v=1489506207\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-CAG-mNIS-Neo and selected with G418. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1063_uptake_large.jpg?v=1489506233\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35258959811,"sku":"(DNA1063-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1065","title":"pLV-Ub-humanNIS-P2A-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-Ub-human NIS-P2A-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-Ub-hNIS-P2A-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: \u003cspan\u003eUbiquitin (Ub) promoter for constitutive gene expression in numerous cell types. \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Human sodium iodide symporter (hNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker: \u003c\/strong\u003eSelf-cleavage peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding human NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1065_map_large.png?v=1489507104\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHelaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-Ub-hNIS-P2A-Puro  and selected with puromycin. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1065_uptake_large.jpg?v=1489507126\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35259615235,"sku":"(DNA1065-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1066","title":"pLV-Ub-mouseNIS-P2A-Neo","description":"\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-Ub-mouse NIS-P2A-Neo\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-Ub-mNIS-P2A-Neo plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: \u003cspan\u003eUbiquitin (Ub) promoter for constitutive gene expression in numerous cell types. \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Mouse sodium iodide symporter (mNIS) and neomycin resistance (Neo)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLinker:\u003c\/strong\u003e Self-cleaving peptide P2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding mouse NIS and neomycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1066_map_large.png?v=1489507826\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-Ub-mNIS-Neo and selected with G418. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1066_uptake_large.jpg?v=1489507848\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35260172099,"sku":"(DNA1066-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1072","title":"pLV-TBG-mouseNIS","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-TBG-mouse NIS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: \u003cspan\u003epHR'-SIN-TBG-mNIS plasmid DNA\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: Liver-specific human thyroxine-binding globulin (TBG) promoter\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene\u003c\/strong\u003e: Mouse sodium iodide symporter (mNIS)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of liver-specific lentiviral vector stocks encoding mouse NIS in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1072_map_7fbf90b1-6e3b-450f-880b-1ecc5389d59d_large.png?v=1489587669\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003e\u003cspan\u003eHepa1-6 hepatoma cells were transduced with LV-TGB-mNIS at various MOI. NIS mediated \u003csup\u003e125\u003c\/sup\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1072_uptake_large.png?v=1489587856\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35316611331,"sku":"(DNA1072-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1036","title":"pLV-SFFV-dogNIS-PGK-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-dog NIS-PGK-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-dNIS-PGK-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. \u003cspan\u003ePGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Dog sodium iodide symporter (dNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding dog NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1036_map_large.png?v=1488991253\" alt=\"plasmid map, dNIS puro\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-dNIS-PGK-Puro and selected with puromycin. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1019_uptake_large.jpg?v=1488904737\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35368995395,"sku":"(DNA1036-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1038","title":"pLV-SFFV-rhesusNIS-PGK-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-SFFV-Rhesus NIS-PGK-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-SFFV-RhNIS-PGK-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. \u003cspan\u003ePGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Rhesus sodium iodide symporter (RhNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan _mce_style=\"color: #ff6600;\"\u003e\u003cstrong\u003eProposed Use\u003c\/strong\u003e\u003c\/span\u003e: For generation of lentiviral vector stocks encoding Rhesus NIS and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1038_map_large.jpg?v=1488993078\" alt=\"plasmid map, RhNIS puro\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-RhNIS-PGK-Puro and selected with puromycin. \u003cspan\u003eNIS mediated \u003c\/span\u003e\u003csup\u003e125\u003c\/sup\u003e\u003cspan\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4,\u003c\/sub\u003e a competitive inhibitor of iodine uptake.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1038_uptake_large.jpg?v=1488993116\" alt=\"Uptake, RhNIS, NIS\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35369033603,"sku":"(DNA1038-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dna1070","title":"pLV-EF1a-rhesusNIS-PGK-Puro","description":"\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eName of plasmid\u003c\/strong\u003e: pLV-EF1a-Rhesus NIS-PGK-Puro\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e: pHR' SIN-EF1a-RhNIS-PGK-Puro plasmid DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAmount of DNA\u003c\/strong\u003e: 20 ug\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter\u003c\/strong\u003e: EF1a: elongation factor-1 alpha promoter for constitutive gene expression in numerous cell types, particularly in cells where other promoters are silenced. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgenes\u003c\/strong\u003e: Rhesus sodium iodide symporter (RhNIS) and puromycin resistance (Puro)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProposed Use\u003c\/strong\u003e: For generation of lentiviral vector stocks encoding Rhesus sodium iodide symporter and puromycin resistance in a triple transfection protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid Map:\u003c\/strong\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1070_map_large.png?v=1489586418\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"display: inline !important;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene Validation: \u003c\/strong\u003eHeLaH1 cells were transduced with\u003cstrong\u003e \u003c\/strong\u003eLV-EF1a-RhNIS-PGK-Puro and selected with puromycin. NIS mediated \u003csup\u003e125\u003c\/sup\u003eI uptake was assayed in the presence or absence of KClO\u003csub\u003e4,\u003c\/sub\u003e a competitive inhibitor of iodine uptake.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/1070_uptake_large.png?v=1489586445\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"20 ug \/ Standard","offer_id":35369070403,"sku":"(DNA1070-STAN)","price":1500.0,"currency_code":"USD","in_stock":true}]}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/collections\/NIS.png?v=1470077647","url":"https:\/\/imanis-life-sciences-4.myshopify.com\/collections\/species-specific-nis.oembed?page=3","provider":"Imanis Life Sciences","version":"1.0","type":"link"}