{"title":"U266B1 Cells","description":"Cell line name contains U266B1","products":[{"product_id":"cl176","title":"U266B1-Fluc-Puro\/eGFP-Neo","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cb\u003eDescription\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eParental U266B1 cells were transduced with \u003c\/span\u003e\u003ca href=\"https:\/\/imanislife.com\/products\/lv-luc2-p2a-puro\/\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eLV-SFFV-Luc2-P2A-Puro (Imanis #LV012)\u003c\/span\u003e\u003c\/a\u003e\u003cspan style=\"font-weight: 400;\"\u003e and \u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003eLV-SFFV-eGFP-P2A-Neo (Imanis #LV067)\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e, which encode the firefly luciferase (Fluc; Luc2) and enhanced green fluorescent protein (eGFP) cDNAs, respectively, under the spleen focus-forming virus (SFFV) promoter and linked to antibiotic resistance (puromycin, neomycin) genes via a P2A cleavage peptide. A high Fluc and eGFP expressing population was generated by selection using puromycin and G418 followed by selection with a methylcellulose based semi-solid medium.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis cell line has been tested for Mycoplasma contamination and is certified mycoplasma free.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe parental U266B1 cell line was licensed and purchased directly from ATCC*.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReplication Competent Lentivirus (RCL) Test \u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e(including a test report) is available for this cell line at an added cost. \u003c\/span\u003e\u003ca href=\"https:\/\/imanislife.com\/services\/sample-analysis\/\"\u003e\u003cb\u003eContact us\u003c\/b\u003e\u003c\/a\u003e\u003cspan style=\"font-weight: 400;\"\u003e to learn more.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCharacterization\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eMorphology\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePhase and fluorescence cell photos taken at 200x magnification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eLuciferase Activity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe indicated number of cells were placed in wells of a 96-well plate. After the addition of 3 mg\/mL d-luciferin, bioluminescence was immediately read using a plate reader. The total bioluminescence (RLU) was plotted as a function of cell number.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eEmGFP Expression\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eU266B1-Fluc-Puro\/eGFP-Neo (green) or parental U266B1 (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry. \u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eSurface Receptor Profiling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eU266B1-Fluc-Puro\/eGFP-Neo (green) cells were stained with isotype control antibody (grey) or antibody specific to human BCMA (A), CD19 (B), CD20 (C), GPRC5D (D), or CD38 (E) and analyzed by flow cytometry. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGrowth Conditions\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eComplete Growth Medium: ATCC-formulated RPMI-1640 supplemented with 15% fetal bovine serum (FBS), 1% Penicillin\/Streptomycin, 3 μg\/mL puromycin, and 1 mg\/mL G418.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThese cells should be grown in the indicated medium and subcultured as needed to maintain a density between 5 x 10\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e5\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e and 2 x 10\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e6\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e cells\/mL. The cells can be passaged by dilution in fresh medium, with occasional passaging using centrifugation to limit the amount of debris in cultures.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eUsage Information\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThese cells are suitable for \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003ein vitro\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e and \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003ein vivo\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e experimentation. The Fluc transgene facilitates \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003ein vivo\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e noninvasive bioluminescent imaging of implanted cells. eGFP is not recommended for in-life imaging, but can be used for post-mortem confirmatory assays. Both Fluc and eGFP facilitate quantitation of cells \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003ein vitro\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThese cells were generated via lentiviral vector transduction. The lentiviral vector used for transduction was a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003ecis\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e-acting effects of the LTR\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003e. Nevertheless, all work with these cells should be performed under biosafety-level 2 (BSL2) conditions by trained personnel. Institutional requirements may permit handling of these cells under BSL1 conditions if certain criteria are met.\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eMiyoshi, H \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003eet al. \u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e(1998). Development of a self-inactivating lentivirus vector. \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003eJournal of Virology\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e 72: 8150-8157. \u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":42741076885685,"sku":"CL176","price":3000.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cl173","title":"U266B1-Fluc-Puro","description":"\u003cp\u003eParental U266B1 cells were transduced with LV-SFFV-Luc2-P2A-Puro (Imanis\u003cbr data-mce-fragment=\"1\"\u003e#LV012) encoding the firefly luciferase (Fluc; Luc2) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin followed by selection with a methylcellulose based semi-solid medium.\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis cell line has been tested for Mycoplasma contamination and is free of Mycoplasma\u003cbr data-mce-fragment=\"1\"\u003especies.\u003c\/p\u003e\n\u003cp\u003e\u003cbr data-mce-fragment=\"1\"\u003eThe parental U266B1 cell line was licensed and purchased directly from ATCC*.\u003cbr data-mce-fragment=\"1\"\u003eReplication Competent Lentivirus (RCL) Test (including a test report) is available for this cell line at an added cost. Contact us to learn more.\u003c\/p\u003e\n\u003cp\u003e\u003cbr data-mce-fragment=\"1\"\u003e*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":42897462919349,"sku":"CL173-STAN","price":2500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cl184","title":"U266B1-Fluc-Puro\/BCMA-KO","description":"\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e$\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e3\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e5\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e00\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eSpecies: Human\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eCell Type: \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eMultiple Myeloma\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eTransgene: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop SCXW159743030 BCX4\"\u003e\u003cspan class=\"TabChar SCXW159743030 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW159743030 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eFirefly luciferase (\u003c\/span\u003e\u003cspan class=\"NormalTextRun SpellingErrorV2Themed SCXW159743030 BCX4\"\u003eFluc\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e; Luc2)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eSelection Gene: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop SCXW159743030 BCX4\"\u003e\u003cspan class=\"TabChar SCXW159743030 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW159743030 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003ePuromycin resistance (Puro)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eKnock-\u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\"\u003eout:\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\"\u003e\u003cspan class=\"TabChar SCXW159743030 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW159743030 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eB-cell maturation antigen (BCMA)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eDescription\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThe \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-Fluc-Puro\/\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA-KO\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e cell line stably expresses firefly luciferase \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eand does not express BCMA. To generate this cell line, U266B1-Fluc-Puro \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003ca class=\"Hyperlink SCXW159743030 BCX4\" href=\"https:\/\/imanislife.com\/products\/nalm6-fluc-puro\/\" rel=\"noreferrer noopener\" target=\"_blank\"\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentStart CommentHighlightPipeRest CommentHighlightRest SCXW159743030 BCX4\" data-ccp-charstyle=\"Hyperlink\"\u003eImanis\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightRest SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e #CL173\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightPipeRest SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e) cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e expression by flow cytometry. Sequencing of the targeted \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e region confirmed genetic knock-out in the selected cell clone. \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis cell line has been tested for mycoplasma contamination and is \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003ecertified\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e mycoplasma free.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis cell line is also available for purchase as a pair with the \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-Fluc-Puro (\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e positive) cell line, for a discounted rate (\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentStart CommentHighlightPipeRest CommentHighlightRest SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003elink\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightPipeRest SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e). \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eCharacterization\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eMorphology\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003ePhase cell photo taken at 200x magnification\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan class=\"SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun EmptyTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan class=\"WACImageContainer NoPadding BlobObject SCXW159743030 BCX4\" role=\"presentation\"\u003e\u003cimg src=\"data:image\/png;base64,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\" alt=\"The image depicts a highly textured surface with numerous small, closely packed, irregularly shaped features, approximately 50 micrometers in size.\n\nAI-generated content may be incorrect.\" class=\"WACImage SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun EmptyTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eLuciferase\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e Expression\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eThe indicated number of cells were placed in wells of a 96-well plate. After the addition of \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e15 mg\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\/mL d-luciferin, bioluminescence was \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eimmediately\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e read using a microplate reader.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"335551550\":6,\"335551620\":6,\"335559739\":0}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan class=\"WACImageContainer NoPadding AttachedToBeginning SCXW159743030 BCX4\" role=\"presentation\"\u003e\u003cimg 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class=\"WACImage SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e Expression\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e-Fluc-Puro\/\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eBCMA-KO\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e cells were stained with an anti-\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e (blue) or isotype control (grey)\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e antibody and analyzed by flow cytometry.\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e (Note: \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003ecells were grown in the presence of \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e-secretase \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\"\u003einhibitor\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e to stabilize any surface BCMA \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\"\u003eexpression.\u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan class=\"SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun EmptyTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan class=\"WACImageContainer NoPadding BlobObject SCXW159743030 BCX4\" role=\"presentation\"\u003e\u003cimg alt=\"The image displays a bar chart comparing the relative abundance of different isotypes, including a-BCMA, with a peak at 101 and a maximum value of 80%.\n\nAI-generated content may be incorrect.\" class=\"WACImage SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Highlight EmptyTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun Underlined SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eSurface\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e Receptor Profiling\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"SCXW159743030 BCX4\"\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eU266B1-\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eFluc-Puro\/\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eBCMA-KO\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e cells were stained with an anti-\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eHuCD19\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e (A), anti-\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eHu\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eCD20 (B), anti-Hu\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eCD38\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e (C), or anti-Hu\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eGPRC5D\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e (D)\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e antibody and analyzed by flow cytometry.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan class=\"SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun EmptyTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan class=\"WACImageContainer NoPadding BlobObject SCXW159743030 BCX4\" role=\"presentation\"\u003e\u003cimg alt=\"The image depicts a series of five graphs, each showing the relative expression levels of various cell markers (CD19, CD20, CD38, GPRC5D) in different samples, with the data represented as percentages and peaks indicating specific antibody binding.\n\nAI-generated content may be incorrect.\" class=\"WACImage SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun EmptyTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eGrowth Conditions\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eComplete Growth Medium: \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eATCC-formulated RPMI-1640\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e supplemented with 15% fetal bovine serum (FBS)\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e and\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e 1% Penicillin\/Streptomycin. \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eFor long-term maintenance (more than two to three weeks) addition of \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e3\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e µg\/mL of puromycin to the media is recommended\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThese cells should be grown in the \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eindicated\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e medium and \u003c\/span\u003e\u003cspan class=\"NormalTextRun SpellingErrorV2Themed SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003esubcultured\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e as needed to \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003emaintain\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e a density between \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e x 10\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun Superscript SCXW159743030 BCX4\" data-fontsize=\"11\" data-ccp-parastyle=\"Normal (Web)\"\u003e5\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e and \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e1.5\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e x 10\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun Superscript SCXW159743030 BCX4\" data-fontsize=\"11\" data-ccp-parastyle=\"Normal (Web)\"\u003e6\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e cells\/\u003c\/span\u003e\u003cspan class=\"NormalTextRun SpellingErrorV2Themed SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003emL.\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e The cells can be passaged by dilution in fresh medium, with occasional passaging using centrifugation to limit the \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eamount\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e of debris in cultures. \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eFor \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eoptimal\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e growth and viability, do a media change every 3-4 days even if the cells are not ready to \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003epassage\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e. \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":true,\"201341983\":0,\"335557856\":16777215,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eUsage Information\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThese cells are suitable for \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-charstyle=\"Emphasis\"\u003ein vitro\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e and \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-charstyle=\"Emphasis\"\u003ein vivo\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e experimentation. \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThe \u003c\/span\u003e\u003cspan class=\"NormalTextRun SpellingErrorV2Themed SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eFluc\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e transgene \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003efacilitates\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-charstyle=\"Emphasis\"\u003ein vivo\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e noninvasive bioluminescent imaging of implanted cells and quantitation of cells \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003ein vitro\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":true,\"201341983\":0,\"335557856\":16777215,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThese cells were generated via lentiviral vector transduction. The lentiviral vector\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003es\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e used for transduction \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003ewere\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e self-inactivating (SIN) vector\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003es\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e in which the viral enhancer and promoter have been \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003edeleted\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-charstyle=\"Emphasis\"\u003ecis\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-acting effects of the LTR\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun Superscript SCXW159743030 BCX4\" data-fontsize=\"11\" data-ccp-parastyle=\"Normal (Web)\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e. Nevertheless, all work with these cells should be performed under biosafety-level 2 (BSL2) conditions by trained personnel. Institutional requirements may \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003epermit\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e handling of these cells under BSL1 conditions if certain criteria are met.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":true,\"201341983\":0,\"335557856\":16777215,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ListContainerWrapper SCXW159743030 BCX4\"\u003e\n\u003col class=\"NumberListStyle1 SCXW159743030 BCX4\" role=\"list\" start=\"1\"\u003e\n\u003cli aria-setsize=\"-1\" data-leveltext=\"%1.\" data-font=\"Arial\" data-listid=\"3\" data-list-defn-props='{\"335552541\":0,\"335559685\":720,\"335559991\":360,\"469769242\":[65533,0],\"469777803\":\"left\",\"469777804\":\"%1.\",\"469777815\":\"hybridMultilevel\"}' data-aria-posinset=\"1\" data-aria-level=\"1\" role=\"listitem\" class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW159743030 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eMiyoshi, H \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eet al. \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e(1998). Development of a self-inactivating lentivirus vector. \u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003eJournal of Virology\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW159743030 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW159743030 BCX4\"\u003e 72: 8150-8157. \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW159743030 BCX4\" data-ccp-props='{\"335559685\":360}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003e*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":52685368295605,"sku":"CL184","price":3500.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cl185","title":"U266B1-Fluc-Puro\/BCMA (mid)","description":"\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e$3500\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eSpecies: Human\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eCell Type: Multiple Myeloma\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eTransgene: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop SCXW96026457 BCX4\"\u003e\u003cspan class=\"TabChar SCXW96026457 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW96026457 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eFirefly luciferase (\u003c\/span\u003e\u003cspan class=\"NormalTextRun SpellingErrorV2Themed SCXW96026457 BCX4\"\u003eFluc\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e; Luc2)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eSelection Gene: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop SCXW96026457 BCX4\"\u003e\u003cspan class=\"TabChar SCXW96026457 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW96026457 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003ePuromycin resistance (Puro)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eKnock-\u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW96026457 BCX4\"\u003edown:\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop ContextualSpellingAndGrammarErrorV2Themed SCXW96026457 BCX4\"\u003e\u003cspan class=\"TabChar SCXW96026457 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW96026457 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eB-cell maturation antigen (BCMA)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eDescription\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThe \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-Fluc-Puro\/\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA (mid)\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e cell line stably expresses firefly luciferase \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eand reduced BCMA levels. To generate this cell line, U266B1-Fluc-Puro \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003ca class=\"Hyperlink SCXW96026457 BCX4\" href=\"https:\/\/imanislife.com\/products\/nalm6-fluc-puro\/\" rel=\"noreferrer noopener\" target=\"_blank\"\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentStart CommentHighlightPipeRest CommentHighlightRest SCXW96026457 BCX4\" data-ccp-charstyle=\"Hyperlink\"\u003eImanis\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e #CL173\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightPipeRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e) cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e expression by flow cytometry. \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis clone was selected based on its reduced expression of BCMA. \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis cell line has been tested for mycoplasma contamination and is \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003ecertified\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e mycoplasma free.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis cell line is also available for purchase as a pair with the \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-Fluc-Puro (\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e positive) cell line, for a discounted rate (\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentStart CommentHighlightPipeRest CommentHighlightRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003elink\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightPipeRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e). \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":52685547602101,"sku":"CL185","price":3500.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/collections\/u266b1-cells.oembed","provider":"Imanis Life Sciences","version":"1.0","type":"link"}