{"product_id":"c1498-fluc-neoegfp-puro","title":"C1498-Fluc-Neo\/eGFP-Puro","description":"This is a cell line derived from the murine acute myeloid leukemia C1498 cell line (ATCC® TIB-49\u003csup\u003eTM\u003c\/sup\u003e). Parental C1498 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide, and 2) LV-eGFP-PGK-Puro (Imanis #LV031) encoding the enhanced green fluorescent protein (eGFP) cDNA under the SFFV promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. A high Fluc- and eGFP-expressing population was generated by selection using G418 and puromycin followed by selection using a methylcellulose based semi-solid medium.","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":15990880731208,"sku":"CL145-STAN","price":3000.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/c1498-fluc-neoegfp-puro","provider":"Imanis Life Sciences","version":"1.0","type":"link"}