{"product_id":"cl016","title":"RG2-hNIS","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eSpecies:\u003c\/strong\u003e Rat\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell type:\u003c\/strong\u003e Glial tumor\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgene:\u003c\/strong\u003e \u003ca href=\"http:\/\/www.imanislife.com\/pages\/what-is-nis\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman sodium iodide symporter (hNIS)\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMedia:\u003c\/strong\u003e DMEM, 10% FBS, 1% Pen\/Strep\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription:\u003c\/strong\u003e RG2-hNIS is a polyclonal population of the rat glial tumor RG2 cell line transduced with \u003ca href=\"http:\/\/www.imanislife.com\/products\/lv001\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eLV-hNIS (LV001)\u003c\/a\u003e encoding the human sodium iodide symporter (hNIS) cDNA under the spleen focus-forming virus (SFFV) promoter.\u003c\/p\u003e\n\u003cp\u003eThe lentiviral vector used is a self-inactivating (SIN) vector in which the viral enhancer and promoter has been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended uses:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eIn vitro\u003c\/em\u003e: This is a high hNIS expressing clone suitable for use as a positive control cell line in I-125 uptake assays to validate NIS expression in your lentiviral transduced cells. \u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eIn vivo\u003c\/em\u003e: This rat glial tumor cell line grows as a glioma after intracranial injection into Fisher 344 rats. Once large it’s growth can be monitored noninvasively using high-resolution 3D SPECT or PET imaging due to disruption of the blood-brain-barrier allowing for radioiodine access to the tumor cells.\u003c\/p\u003e\n\u003cp\u003eNote: disrupting the blood-brain barrier with mannitol treatment may allow for NIS imaging at early stages of tumor formation. \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eMorphology:\u003c\/strong\u003e Low- and high-density cell morphology (200x)\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/Cell_Photos_for_COA_CL016_large.jpg?6798270302640773195\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNIS Function Assay (Iodine Uptake):\u003c\/strong\u003e Cells were incubated with \u003csup\u003e125\u003c\/sup\u003eI for 1h in the presence or absence of KClO\u003csub\u003e4\u003c\/sub\u003e, an inhibitor of iodine uptake. Radioiodine concentrated within the cells was measured with a gamma counter.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/I125_uptake_for_COA_CL016_large.jpg?11585960446810577198\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReferences on NIS imaging\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. Fruthwirth et al. A whole body dual modality radionuclide optical strategy for preclinical imaging of metastasis and heterogeneous treatment responses in different. microenvironments. \u003ca href=\"http:\/\/jnm.snmjournals.org\/content\/55\/4\/686.long\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eJ. Nucl. Med 2014. 55(4): 686-94.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Penheiter et al. The sodium iodide symporter (NIS) as an imaging reporter for gene, viral and cell-based therapies. \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22263922\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cspan class=\"jrnl\" title=\"Current gene therapy\"\u003eCurr Gene Ther\u003c\/span\u003e\u003cspan\u003e. 2012, 12(1):33-47.\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":15996325265480,"sku":"CL016-STAN","price":1500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl016","provider":"Imanis Life Sciences","version":"1.0","type":"link"}