{"product_id":"cl147","title":"A20-Fluc-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eSpecies:\u003c\/strong\u003e\u003cspan\u003e Mouse\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell type:\u003c\/strong\u003e\u003cspan\u003e Lymphoma\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes:\u003c\/strong\u003e\u003cspan\u003e Firefly Luciferase (Fluc)\u003c\/span\u003e and puromycin resistance (Puro) for selection with puromycin\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMedia:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eRPMI, 10% FBS, 1% Pen\/Strep\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis is a cell line derived from the murine lymphoma A20 cell line (ATCC® TIB-208\u003csup\u003eTM\u003c\/sup\u003e). Parental A20 cells were transduced with LV-Fluc-P2A-Puro (Imanis #LV012) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin followed by selection using a methylcellulose based semi-solid medium. The lentiviral vector is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR\u003csup\u003e1\u003c\/sup\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell Line Authentication:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe parental A20 cell line was authenticated and certified free of interspecies cross contamination by STR profiling.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended uses:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThese cells are suitable for \u003cem\u003ein vitro \u003c\/em\u003eand \u003cem\u003ein vivo \u003c\/em\u003eexperimentation.\u003c\/p\u003e\n\u003cp\u003eA20 cells form tumors and metastases at multiple sites, including the liver, lymph nodes, spleen, bone marrow, ovaries, and peritoneal cavity, upon implantation into syngeneic Balb\/c mice\u003csup\u003e6\u003c\/sup\u003e.\u003c\/p\u003e\n\u003cp\u003eThe Fluc transgene facilitates \u003cem\u003ein vivo \u003c\/em\u003enoninvasive bioluminescent imaging of implanted cells. Fluc is immunogenic and may cause tumor rejection in immunocompetent mice. For the most consistent results, immunocompromised mice are recommended for studies.  \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eCell morphology:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLow- and high-density cell morphology (200x)\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/A20_Morphology_large.jpg?v=1510348446\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLuciferase Expression\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe indicated number of cells were placed in wells of a 96-well plate. After the addition of 3 mg\/mL d-luciferin, the plate was immediately imaged using an IVIS Spectrum. The total flux (photons\/sec) was plotted as a function of cell number.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/files\/A20_Luciferase_Assay_large.jpg?v=1510348485\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":15990856482888,"sku":"CL147-STAN","price":2500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl147","provider":"Imanis Life Sciences","version":"1.0","type":"link"}