{"product_id":"cl150","title":"Nalm6-Fluc-Neo\/eGFP-Puro","description":"\u003cdiv class=\"page\" title=\"Page 1\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cspan\u003eThis is a cell line derived from the human B cell precursor leukemia Nalm6 cell line (ATCC® CRL-3273\u003c\/span\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003cspan\u003e). Parental Nalm6 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus- forming virus (SFFV) promoter and linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide and 2) LV- eGFP-PGK-Puro (Imanis #LV031) encoding the enhanced green fluorescent protein (eGFP) cDNA under the SFFV promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. A high Fluc and eGFP expressing population was generated by selection using G418 and puromycin followed by selection using a methylcellulose based semi-solid medium. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":44263915843,"sku":"CL150-STAN","price":3000.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl150","provider":"Imanis Life Sciences","version":"1.0","type":"link"}