{"product_id":"cl174","title":"RPMI-8226-Fluc-Puro","description":"\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eParental RPMI-8226 cells were transduced with \u003c\/span\u003e\u003ca href=\"https:\/\/imanislife.com\/products\/lv-luc2-p2a-puro\/\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eLV-SFFV-Luc2-P2A-Puro (Imanis #LV012)\u003c\/span\u003e\u003c\/a\u003e\u003cspan style=\"font-weight: 400;\"\u003e encoding the firefly luciferase (Fluc; Luc2) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin followed by selection with a methylcellulose based semi-solid medium.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis cell line has been tested for \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003eMycoplasma\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e contamination and is free of \u003c\/span\u003e\u003ci\u003e\u003cspan style=\"font-weight: 400;\"\u003eMycoplasma\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-weight: 400;\"\u003e species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe parental RPMI-8226 cell line was licensed and purchased directly from ATCC*. \u003c\/span\u003e\u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":43135483936949,"sku":"CL174","price":2500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl174","provider":"Imanis Life Sciences","version":"1.0","type":"link"}