{"product_id":"cl181","title":"Raji-Fluc-Puro\/CD19-KO","description":"\u003cdiv style=\"mso-element: comment-list;\"\u003e\n\u003cp\u003eThe Raji-Fluc-Puro\/CD19-KO cell line stably expresses firefly luciferase and does not express CD19. To generate this cell line, Raji-Fluc-Puro (\u003ca href=\"https:\/\/imanislife.com\/products\/raji-fluc-puro\/\"\u003eImanis #CL161\u003c\/a\u003e\u003cu\u003e)\u003c\/u\u003e cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human CD19. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for CD19 expression by flow cytometry. Sequencing of the targeted CD19 region confirmed heterologous genetic knock-out in the selected cell clone.\u003c\/p\u003e\n\u003cp\u003eThis cell line has been tested for mycoplasma contamination and is certified mycoplasma free.\u003c\/p\u003e\n\u003cp\u003eThe parental Raji cell line was licensed and purchased directly from ATCC*.\u003c\/p\u003e\n\u003cp\u003eThis cell line is also available for purchase as a pair with the Raji-Fluc-Puro (CD19 positive) cell line, for a discounted rate (\u003ca href=\"https:\/\/imanislife.com\/collections\/cell-lines\/cd19\/\"\u003elink\u003c\/a\u003e).\u003c\/p\u003e\n\u003chr class=\"msocomoff\" align=\"left\" size=\"1\" width=\"33%\"\u003e\n\u003c!--[endif]--\u003e\n\u003cdiv style=\"mso-element: comment;\"\u003e\n\u003c!-- [if !supportAnnotations]--\u003e\n\u003cdiv id=\"_com_1\" class=\"msocomtxt\"\u003e\n\u003c!--[endif]--\u003e\u003cspan style=\"mso-comment-author: 'Rianna Vandergaast'; mso-comment-providerid: AD; mso-comment-userid: 'S::vandergaast\\.rianna\\@imanislife\\.com::e78267c4-30da-4740-afd1-63997f420c96';\"\u003e\u003c!-- [if !supportAnnotations]--\u003e\u003ca name=\"_msocom_1\"\u003e\u003c\/a\u003e\u003c!--[endif]--\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan style=\"font-size: 8.0pt;\"\u003e\u003cspan style=\"mso-special-character: comment;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":51438527250613,"sku":"CL181","price":3500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl181","provider":"Imanis Life Sciences","version":"1.0","type":"link"}