{"product_id":"cl185","title":"U266B1-Fluc-Puro\/BCMA (mid)","description":"\u003cdiv class=\"OutlineElement Ltr SCXW159743030 BCX4\"\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e$3500\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eSpecies: Human\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eCell Type: Multiple Myeloma\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eTransgene: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop SCXW96026457 BCX4\"\u003e\u003cspan class=\"TabChar SCXW96026457 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW96026457 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eFirefly luciferase (\u003c\/span\u003e\u003cspan class=\"NormalTextRun SpellingErrorV2Themed SCXW96026457 BCX4\"\u003eFluc\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e; Luc2)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eSelection Gene: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop SCXW96026457 BCX4\"\u003e\u003cspan class=\"TabChar SCXW96026457 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW96026457 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003ePuromycin resistance (Puro)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eKnock-\u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW96026457 BCX4\"\u003edown:\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"TabRun IPSelectionBlob BlobObject DragDrop ContextualSpellingAndGrammarErrorV2Themed SCXW96026457 BCX4\"\u003e\u003cspan class=\"TabChar SCXW96026457 BCX4\"\u003e \u003c\/span\u003e\u003cspan class=\"TabLeaderChars SCXW96026457 BCX4\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eB-cell maturation antigen (BCMA)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003eDescription\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThe \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-Fluc-Puro\/\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA (mid)\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e cell line stably expresses firefly luciferase \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eand reduced BCMA levels. To generate this cell line, U266B1-Fluc-Puro \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003ca class=\"Hyperlink SCXW96026457 BCX4\" href=\"https:\/\/imanislife.com\/products\/nalm6-fluc-puro\/\" rel=\"noreferrer noopener\" target=\"_blank\"\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentStart CommentHighlightPipeRest CommentHighlightRest SCXW96026457 BCX4\" data-ccp-charstyle=\"Hyperlink\"\u003eImanis\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e #CL173\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightPipeRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e) cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e expression by flow cytometry. \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis clone was selected based on its reduced expression of BCMA. \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis cell line has been tested for mycoplasma contamination and is \u003c\/span\u003e\u003cspan class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003ecertified\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e mycoplasma free.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eThis cell line is also available for purchase as a pair with the \u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eU266B1\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e-Fluc-Puro (\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003eBCMA\u003c\/span\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e positive) cell line, for a discounted rate (\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"none\" lang=\"EN-US\" class=\"TextRun Underlined SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentStart CommentHighlightPipeRest CommentHighlightRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003elink\u003c\/span\u003e\u003c\/span\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun CommentHighlightPipeRest SCXW96026457 BCX4\" data-ccp-parastyle=\"Normal (Web)\"\u003e). \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props='{\"134233117\":true,\"134233118\":false,\"201341983\":0,\"335559739\":0,\"335559740\":276}'\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"OutlineElement Ltr SCXW96026457 BCX4\"\u003e\n\u003cp class=\"Paragraph SCXW96026457 BCX4\"\u003e\u003cspan data-contrast=\"auto\" lang=\"EN-US\" class=\"TextRun SCXW96026457 BCX4\"\u003e\u003cspan class=\"NormalTextRun SCXW96026457 BCX4\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"EOP SCXW96026457 BCX4\" data-ccp-props=\"{}\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":52685547602101,"sku":"CL185","price":3500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl185","provider":"Imanis Life Sciences","version":"1.0","type":"link"}