{"product_id":"cl188","title":"Nalm6-Fluc-Puro\/CD19-KO\/HuCD20-Neo (high)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eDescription\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0in; line-height: 115%;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003eThe Nalm6-Fluc-Puro\/CD19-KO\/HuCD20-Neo (high) cell line stably expresses firefly luciferase and high levels of human CD20, but does not express CD19. To generate this cell line, Nalm6-Fluc-Puro\/CD19-KO cells (\u003ca style=\"mso-comment-reference: RV_1; mso-comment-date: 20251104T0814;\"\u003eImanis #CL18\u003c\/a\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003e) were transduced with a human CD20-encoding lentivirus. A high HuCD20 expressing population was generated by selection using a methylcellulose based semi-solid medium. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0in; line-height: 115%;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003eThis cell line has been tested for mycoplasma contamination and is certified mycoplasma free.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0in; line-height: 115%;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003eThe parental Nalm6 cell line was licensed and purchased directly from ATCC*.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0in; line-height: 115%;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003e*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eGrowth Conditions\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eComplete Growth Medium: RPMI-1640 supplemented with 15% fetal bovine serum (FBS), 10 mM HEPES, and 1% Penicillin\/Streptomycin. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eFor long-term maintenance (more than two to three weeks) addition of 1 µg\/mL of puromycin and 1 mg\/mL G418 can be added to the media. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height: 115%; background: white;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif; color: black; mso-color-alt: windowtext;\"\u003eThese cells should be grown in the indicated medium and subcultured as needed to maintain a density between 7 x 10\u003csup\u003e5\u003c\/sup\u003e and 3 x 10\u003csup\u003e6\u003c\/sup\u003e cells\/mL. The cells can be passaged by dilution in fresh medium, with occasional passaging using centrifugation to limit the amount of debris in cultures.\u003cspan style=\"mso-spacerun: yes;\"\u003e  \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eUsage Information\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"line-height: 115%; background: white;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif; color: black; mso-color-alt: windowtext;\"\u003eThese cells are suitable for \u003cem\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003ein vitro\u003c\/span\u003e\u003c\/em\u003e and \u003cem\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003ein vivo\u003c\/span\u003e\u003c\/em\u003e experimentation. The Fluc transgene facilitates \u003cem\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003ein vivo\u003c\/span\u003e\u003c\/em\u003e noninvasive bioluminescent imaging of implanted cells and quantitation of cells \u003ci\u003ein vitro\u003c\/i\u003e.\u003c\/span\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height: 115%; background: white;\"\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif; color: black; mso-color-alt: windowtext;\"\u003eThese cells were generated via lentiviral vector transduction. The lentiviral vectors used for transduction were self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003ecis\u003c\/span\u003e\u003c\/em\u003e-acting effects of the LTR\u003csup\u003e1\u003c\/sup\u003e. Nevertheless, all work with these cells should be performed under biosafety-level 2 (BSL2) conditions by trained personnel. Institutional requirements may permit handling of these cells under BSL1 conditions if certain criteria are met.\u003c\/span\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoListParagraph\" style=\"margin-left: .25in; mso-add-space: auto; text-indent: -.25in; mso-list: l0 level1 lfo1;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: Arial;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1.\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e     \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eMiyoshi, H \u003ci\u003eet al. \u003c\/i\u003e(1998). Development of a self-inactivating lentivirus vector. \u003ci\u003eJournal of Virology\u003c\/i\u003e 72: 8150-8157. \u003c\/span\u003e\u003c!-- [if !supportAnnotations]--\u003e\u003c!--[endif]--\u003e\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan style=\"font-size: 8.0pt;\"\u003e\u003cspan style=\"mso-special-character: comment;\"\u003e\u003c!-- [if !supportAnnotations]--\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"mso-element: comment-list;\"\u003e\n\u003cdiv style=\"mso-element: comment;\"\u003e\n\u003c!-- [if !supportAnnotations]--\u003e\n\u003cdiv id=\"_com_1\" class=\"msocomtxt\"\u003e\n\u003c!--[endif]--\u003e\u003cspan style=\"mso-comment-author: 'Rianna Vandergaast'; mso-comment-providerid: AD; mso-comment-userid: 'S::vandergaast\\.rianna\\@imanislife\\.com::e78267c4-30da-4740-afd1-63997f420c96';\"\u003e\u003c!-- [if !supportAnnotations]--\u003e\u003ca name=\"_msocom_1\"\u003e\u003c\/a\u003e\u003c!--[endif]--\u003e\u003c\/span\u003e\u003c!-- [if !supportAnnotations]--\u003e\n\u003c\/div\u003e\n\u003c!--[endif]--\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"mso-element: comment-list;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003c!--[endif]--\u003e\n\u003cdiv style=\"mso-element: comment;\"\u003e\n\u003c!-- [if !supportAnnotations]--\u003e\n\u003cdiv id=\"_com_1\" class=\"msocomtxt\"\u003e\n\u003c!--[endif]--\u003e\u003cspan style=\"mso-comment-author: 'Rianna Vandergaast'; mso-comment-providerid: AD; mso-comment-userid: 'S::vandergaast\\.rianna\\@imanislife\\.com::e78267c4-30da-4740-afd1-63997f420c96';\"\u003e\u003c!-- [if !supportAnnotations]--\u003e\u003ca name=\"_msocom_1\"\u003e\u003c\/a\u003e\u003c!--[endif]--\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan style=\"font-size: 8.0pt;\"\u003e\u003cspan style=\"mso-special-character: comment;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":51992076517557,"sku":"CL188","price":3500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl188","provider":"Imanis Life Sciences","version":"1.0","type":"link"}