{"product_id":"cl191","title":"K562-Fluc-Puro\/HuCD20-Neo","description":"\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSpecies: Human\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCell Type: Chronic Myelogenous Leukemia\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eTransgenes: \u003c\/span\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eFirefly luciferase (Fluc; Luc2)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eHuman CD20 (HuCD20)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSelection Gene: \u003c\/span\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003ePuromycin resistance (Puro)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eNeomycin resistance (Neo)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003cspan\u003e: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe K562-Fluc-Puro\/HuCD20-Neo cell line stably expresses firefly luciferase and human CD20. The cells are designed for \u003c\/span\u003e\u003cspan\u003ein vitro \u003c\/span\u003e\u003cspan\u003eand \u003c\/span\u003e\u003cspan\u003ein vivo \u003c\/span\u003e\u003cspan\u003estudies of CD20. Parental K562-Fluc-Puro cells can be used as a negative control.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eParental K562-Fluc-Puro cells (Imanis #CL171) cells were transduced with a human CD20-encoding lentivirus. A high HuCD20-expressing population was generated by selection using a methylcellulose based semi-solid medium. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThis cell line has been tested for mycoplasma contamination and is certified mycoplasma free.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe parental K562 cell line was licensed and purchased directly from ATCC*.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReplication Competent Lentivirus (RCL) Test \u003c\/span\u003e\u003cspan\u003e(including a test report) is available for this cell line at an added cost. \u003c\/span\u003e\u003ca href=\"https:\/\/imanislife.com\/services\/sample-analysis\/\"\u003e\u003cspan\u003eContact us\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e to learn more.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCharacterization\u003c\/span\u003e\u003cspan\u003e: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eMorphology\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cimg alt=\"A close-up of a microscope\n\nDescription automatically generated\" src=\"https:\/\/lh7-rt.googleusercontent.com\/docsz\/AD_4nXdIlPfl_4aMjtGDTCDyeot-43UcI79Dw9vIw7Tg07_zL_sb62pJWOJuW25oiD7_pBxONbJNdDRMQtQSXdXwqC4eJjKHSDOIxjy8j9P7i_HCn5ZcafjDGW7a_e5ud18EXd1uhDd-GuiqMUN13wouJpVvWWS00CqukA26VF_qvRx5mfsBLRsSLXg?key=ebvvV-_CxA1DeZtdF-FzyXaJ\" width=\"340\" height=\"256\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePhase cell photo taken at 200x magnification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eLuciferase Expression\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe indicated number of cells were placed in wells of a 96-well plate. After the addition of 15 mg\/mL d-luciferin, bioluminescence was immediately read using a microplate reader.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/lh7-rt.googleusercontent.com\/docsz\/AD_4nXegq7hlb0Bsj9PwgtohTBfvBLu-kPT3n41xAb05dcCQ2Fawjxzp2mbSV2nzkPxD5o8EOPAf6FE1rvlROs-gfQ9izGmlv7ul5JnMhuu8bSP94suKJ9ebumawFCSuVSa9CLI8M0sxXL488WloTLej_NMVsFoUKsB1sHC8avZuMSkr3pfuwI7Su00?key=ebvvV-_CxA1DeZtdF-FzyXaJ\" width=\"227\" height=\"232.5484822058885\"\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCD20 Expression\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eK562-Fluc-Puro\/HuCD20-Neo cells were stained with an anti-HuCD20 (blue) or isotype control (grey) antibody and analyzed by flow cytometry.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cimg alt=\"A diagram of a blue and grey scale\n\nDescription automatically generated with medium confidence\" src=\"https:\/\/lh7-rt.googleusercontent.com\/docsz\/AD_4nXevlVMfmOZROmy5s36GVC1hnGvBSJvSfTrH0kXuUiDVdv-20bNc-V4n7f_sQnIHXQznLIjCZmmqunOJKc_HA7k8O_CwxOIATXGOiaZMYBxa4iZZn3B1D3_-CABDMaxrpoitV6JKnaAQ-SmoQsIztByyIxn8mE6waP8roXGcCb_V8z6UzAXcAw?key=ebvvV-_CxA1DeZtdF-FzyXaJ\" width=\"420\" height=\"192\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSurface Receptor Profiling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eK562-Fluc-Puro\/HuCD20-Neo cells were stained with an anti-HuBCMA (A), anti-CD19 (B), anti-HuGPRC5D (C), or anti-HuCD38 (D) antibody and analyzed by flow cytometry.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cimg alt=\"A graph of a number of blue and gray lines\n\nDescription automatically generated with medium confidence\" src=\"https:\/\/lh7-rt.googleusercontent.com\/docsz\/AD_4nXedv996ElwsVZJwNYuXFy0umr45pkkYHN2mWBBDosJh43slk98kfygdUTvb-QkHcF9tZkjmQ550gpUhXcxEOeHbc8xLmnY3lWS1PxG8tyblFA45v_9CYpeOXKEhieMgpGJV5sTwEVTCaqGoS7_wqilB_IuxmYu-Lre4ITtVaMTNN9KyN2OsHb0?key=ebvvV-_CxA1DeZtdF-FzyXaJ\" width=\"507\" height=\"388\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eGrowth Conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eComplete Growth Medium: Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 15% fetal bovine serum (FBS) and 1% Penicillin\/Streptomycin. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFor long-term maintenance (more than two to three weeks) addition of 6 µg\/mL of puromycin and 1 mg\/mL of G418 to the media is recommended to maintain high Fluc and HuCD20 expression. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThese cells should be grown in the indicated medium and subcultured as needed to maintain a density between 5 x 10\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e and 2 x 10\u003c\/span\u003e\u003cspan\u003e6\u003c\/span\u003e\u003cspan\u003e cells\/mL. The cells can be passaged by dilution in fresh medium, with occasional passaging using centrifugation to limit the amount of debris in cultures.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eUsage Information\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThese cells are suitable for \u003c\/span\u003e\u003cspan\u003ein vitro\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003ein vivo\u003c\/span\u003e\u003cspan\u003e experimentation. The Fluc transgene facilitates \u003c\/span\u003e\u003cspan\u003ein vivo\u003c\/span\u003e\u003cspan\u003e noninvasive bioluminescent imaging of implanted cells and quantitation of cells \u003c\/span\u003e\u003cspan\u003ein vitro\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThese cells were generated via lentiviral vector transduction. The lentiviral vectors used for transduction were self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003c\/span\u003e\u003cspan\u003ecis\u003c\/span\u003e\u003cspan\u003e-acting effects of the LTR\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e. Nevertheless, all work with these cells should be performed under biosafety-level 2 (BSL2) conditions by trained personnel. Institutional requirements may permit handling of these cells under BSL1 conditions if certain criteria are met.\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli dir=\"ltr\" aria-level=\"1\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eMiyoshi, H \u003c\/span\u003e\u003cspan\u003eet al. \u003c\/span\u003e\u003cspan\u003e(1998). Development of a self-inactivating lentivirus vector. \u003c\/span\u003e\u003cspan\u003eJournal of Virology\u003c\/span\u003e\u003cspan\u003e 72: 8150-8157. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Imanis Life Sciences","offers":[{"title":"Frozen \/ Standard","offer_id":44781859864757,"sku":"CL191","price":3000.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/cl191","provider":"Imanis Life Sciences","version":"1.0","type":"link"}