{"product_id":"lv039","title":"LV-DsRed-PGK-Puro","description":"\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-titer purified LV-DsRed-PGK-Puro stocks\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePromoters:\u003c\/strong\u003e Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models. Phosphoglycerate kinase promoter for lower level constitutive expression in numerous cell types.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransgenes: \u003c\/strong\u003eRed fluorescent protein (DsRed) and the puromycin resistance gene (Puro)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvelope\/Tropism: \u003c\/strong\u003eVSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eConstruct: \u003c\/strong\u003eThis is a ready-to-use lentivirus preparation. The virus encodes the red fluorescent protein (DsRed) cDNA under control of the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (puro) under the phosphoglycerate kinase (PGK) promoter (see below). The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without \u003cem\u003ecis\u003c\/em\u003e-acting effects of the LTR (Miyoshi et al., J Virol. 1998).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter: \u003c\/strong\u003eApproximately 2e7 TU\/mL (by qPCR titration of transduced HeLa H1 cells)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProposed Use: \u003c\/strong\u003e\u003cem\u003eIn vitro \u003c\/em\u003etransduction of primary cells and cell lines.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-50\"\u003e\n\u003cp\u003e\u003cstrong\u003eVector Map:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV039_construct_large.png?v=1478715161\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFluorescence Expression\u003c\/strong\u003e\u003cstrong\u003e: \u003c\/strong\u003eHeLaH1 cells were transduced with LV-DsRed-PGK-Puro (MOI = 10) and after three days the cells were amplified under puromycin selection. The transduced cells (red) and untransduced control cells (grey) were fixed with paraformaldehyde and analyzed by flow cytometry.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV039_Flow_medium.png?v=1478715163\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Imanis Life Sciences","offers":[{"title":"0.25 mL \/ Standard","offer_id":15996334014536,"sku":"LV039-S-STAN","price":245.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL \/ Standard","offer_id":15996334571592,"sku":"LV039-L-STAN","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0683\/0295\/products\/LV039_construct.png?v=1478715161","url":"https:\/\/imanis-life-sciences-4.myshopify.com\/products\/lv039","provider":"Imanis Life Sciences","version":"1.0","type":"link"}