Imanis Life Sciences

Menu

VSV-deltaM51-NIS

  • 0.25 mL / Standard (OV2005) $ 550

Ready to use oncolytic VSV-M51-NIS

  • Description: Live replication competent Indiana strain of Vesicular Stomatitis Virus (VSV) encoding the human sodium iodide symporter (NIS) inserted as an additional transcription unit upstream of the polymerase (L) gene. The matrix protein is deleted for the Met residue at position 51.
  • Strain: Lab adapted Indiana strain VSV
  • Transgene: Human NIS, upstream of L
  • Biosafety Level: 2
  • Reference: Goel et al. Blood, 2007; 110 (7):2342-2350.
  • Titer: Standard (clarified supernatant, 3e5 to 1e6 TCID50/ml).
  • Propagation methodInoculate producer cell (e.g. Vero or BHK) at MOI of 0.02 for 2-3 hours. Harvest supernatant when 80-90% of cell monolayer is showing cytopathic effects (CPE).
  • Proposed Use: Ready to use for generation of new viral stocks and in cell killing assays.
  • Recommended reagent: Anti-VSV antibody (REA005)
  • USDA permit required for shipping? Yes. Click here to fill out Application Form VS 16-3 to obtain a USDA APHIS VS 16-6 or 16-6A permit.

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

      What people say about Imanis

      Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

      – Dr. Raymond Hickey, Mayo Clinic

      Learning Resources