Imanis Life Sciences

Menu

What packaging system should I use with your plasmids to generate lentivirus?

Our lentiviruses are 2nd generation; our lentiviral transfer plasmids are meant to be used in a 3 plasmid 2nd generation packaging system:

  1. Lentiviral transfer plasmid – the lentiviral plasmid encoding your transgene (ie. pLV-SFFV-hNIS)
  2. Packaging plasmid - encoding Gag, Pol, Rev, and Tat. Tat is essential for 2nd generation packaging.
  3. Envelope plasmid – encoding the envelope protein (usually VSV-G)

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Learning Resources