What packaging system should I use with your plasmids to generate lentivirus?

Our lentiviruses are 2nd generation; our lentiviral transfer plasmids are meant to be used in a 3 plasmid 2nd generation packaging system:

  1. Lentiviral transfer plasmid – the lentiviral plasmid encoding your transgene (ie. pLV-SFFV-hNIS)
  2. Packaging plasmid - encoding Gag, Pol, Rev, and Tat. Tat is essential for 2nd generation packaging.
  3. Envelope plasmid – encoding the envelope protein (usually VSV-G)
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