Description

Type: (-) ssRNA enveloped rhabdovirus

Strain: Lab-adapted Indiana strain

Tropism: The vesicular stomatitis virus envelope glycoprotein (G) mediates binding to many cellular receptors (major receptor being LDLR and LDL-like receptors) giving the virus a very broad tropism (most mammalian cell types)

Gene Map: (Arrows indicate transgene insertion sites)

 

Proposed Uses

Standard titer stocks are ready to use in cell killing assays or for generation of new viral stocks. High-titer purified stocks are ready for immediate use in in vivo studies.

Protocol Tips

Propagation: Inoculate producer cells (e.g. Vero or BHK-21 cells) at an MOI of 0.005 for 1 hour. Collect culture supernatant when 80-90% of the cell monolayer is showing cytopathic effects (CPE, ~48 hours). Clarify the supernatant by low-speed centrifugation. Freeze and store aliquots at or below -70^oC.

Viral Titration: Titer virus by TCID₅₀/mL endpoint dilution assay. Score CPE positive cells based on the presence of dead cells 3 days after inoculation. Alternatively, titer viruses by plaque assay and crystal violet staining. Vero or BHK-21 cells can be used for TCID₅₀ titrations. Vero cells should not be used for titration of recombinant viruses that contain a human interferon (hIFN) transgene as Vero cells are sensitive to antiviral effects of type I IFN.

Regulatory Information

Biosafety Level: 2

US shipping license required? Yes. Please visit the USDA website to apply.