- Default Title (CL139) $ 3,000
This is a population derived from the melanoma B16F10 cell line (ATCC® CRL-6475TM). Parental B16F10 cells were transduced with LV-Luc2-P2A-hNIS (Imanis #LV023) encoding the firefly luciferase (Luc2; Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the human sodium iodide symporter (hNIS) cDNA via a P2A cleavage peptide. High Fluc and hNIS expressing cells were selected using a methylcellulose-based semi solid medium. The lentiviral vector is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR1.