Species: Mouse

Cell type: Mammary carcinoma

Transgenes: Murine sodium iodide symporter (mNIS) with puromycin resistance (Puro) for selection with puromycin

Media: RPMI, 10% FBS, 1% Pen/Strep, 2 μg/mL puromycin

Description: 4T1-mNIS-Neo/eGFP-Puro is a polyclonal population of the murine mammary carcinoma cell line 4T1 (ATCC® CRL-2539™) transduced with LV-mNIS-PGK-Puro (LV022) encoding murine sodium iodide symporter (mNIS) cDNA under the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (Puro) under control of the PGK promoter.

The lentiviral vector used is a self-inactivating (SIN) vector in which the viral enhancer and promoter has been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).

Cell Line Authentication: The parental 4T1 cell line was authenticated and cells are certified free of interspecies cross-contamination by short tandem repeat (STR) profiling with 27 STR loci.

Recommended uses:

In vitro: This is a high mNIS expressing cell line suitable for use as a positive control cell line in iodine uptake and fluorescence assays to verify NIS expression in your lentiviral transduced cells.

In vivo: 4T1 cells form tumors post implantation into mice. The in vivo growth of these tumors can be monitored using noninvasive, high-resolution 3D PET/SPECT imaging.

Morphology: Low- and high-density cell morphology (200x)

Transgene Validation:

NIS function: NIS mediated iodine uptake assay in the presence or absence of KClO4, a competitive inhibitor of uptake.