- Default Title (CL138) $ 2,500
This is a cell line derived from the human B cell precursor leukemia Nalm6 cell line (ATCC® CRL-3273TM). Parental Nalm6 cells were transduced with LV-eGFP-PGK-Puro (Imanis #LV031) encoding the enhanced green fluorescent protein (eGFP) cDNA under the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. A high eGFP expressing population was generated by selection using puromycin followed by selection using a methylcellulose based semi-solid medium. The lentiviral vector is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR1.