Nalm6-Fluc-Puro

This is a cell line derived from the human B cell precursor leukemia Nalm6 cell line (ATCC® CRL-3273TM). Parental Nalm6 cells were transduced with LV-Fluc-P2A-Puro (Imanis #LV012) encoding the firefly luciferase (Fluc) cDNA under the spleen focus- forming virus (SFFV) promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin followed by selection using a methylcellulose based semi-solid medium.