Daudi-Fluc-eGFP
- Frozen / Standard (CL158-STAN) $ 3,000
This is a cell line derived from the human B-lymphoblast Daudi cell line (ATCC® CCL-213TM). Parental Daudi cells were transduced with LV-SFFV-Luc2-P2A-EmGFP (Imanis #LV050) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the emerald green fluorescent protein (eGFP) via a P2A cleavage peptide. Selection was performed using a methylcellulose-based semi-solid medium. The lentiviral vector is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR1.
