U266B1-Fluc-Puro

Parental U266B1 cells were transduced with LV-SFFV-Luc2-P2A-Puro (Imanis
#LV012) encoding the firefly luciferase (Fluc; Luc2) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin followed by selection with a methylcellulose based semi-solid medium.

This cell line has been tested for Mycoplasma contamination and is free of Mycoplasma
species.

The parental U266B1 cell line was licensed and purchased directly from ATCC*.
Replication Competent Lentivirus (RCL) Test (including a test report) is available for this cell line at an added cost. Contact us to learn more.

*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.