RPMI-8226-Fluc-EmGFP

Parental RPMI-8226 cells were transduced with LV-SFFV-Luc2-P2A-EmGFP (Imanis #LV050), encoding the firefly luciferase (Fluc; Luc2) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the emerald green fluorescent protein (EmGFP) cDNA via a P2A cleavage peptide. A high Fluc and eGFP expressing population was generated by selection using a methylcellulose based semi-solid medium.

This cell line has been tested for Mycoplasma contamination and is certified mycoplasma free.

The parental RPMI-8226 cell line was licensed and purchased directly from ATCC*.

Replication Competent Lentivirus (RCL) Test (including a test report) is available for this cell line at an added cost. Contact us to learn more.

*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.