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Raji-Fluc-Puro/CD19-KO

The Raji-Fluc-Puro/CD19-KO cell line stably expresses firefly luciferase and does not express CD19. To generate this cell line, Raji-Fluc-Puro (Imanis #CL161) cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human CD19. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for CD19 expression by flow cytometry. Sequencing of the targeted CD19 region confirmed heterologous genetic knock-out in the selected cell clone.

This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.

The parental Raji cell line was licensed and purchased directly from ATCC*.

This cell line is also available for purchase as a pair with the Raji-Fluc-Puro (CD19 positive) cell line, for a discounted rate (link).

Why Choose Imanis?

With quality reagents, services, and support, we have you covered at all stages of your study. Our expert scientists can help you select the best reagents for your needs and offer tips to optimize your experiments and achieve superior results. Our wide-range of reporter gene and oncolytic virus reagents undergo rigorous quality control testing so you can be confident in the quality of the products you receive. And with our competitive prices, you can complete your study for less.

  • “Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine.” – Dr. Raymond Hickey, Mayo Clinic

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