$3500 

Species: Human 

Cell Type: Multiple Myeloma 

Transgene: Firefly luciferase (Fluc; Luc2) 

Selection Gene: Puromycin resistance (Puro) 

Knock-down: B-cell maturation antigen (BCMA) 

Description:  

The U266B1-Fluc-Puro/BCMA (mid) cell line stably expresses firefly luciferase and reduced BCMA levels. To generate this cell line, U266B1-Fluc-Puro (Imanis #CL173) cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human BCMA. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for BCMA expression by flow cytometry. This clone was selected based on its reduced expression of BCMA.   

This cell line has been tested for mycoplasma contamination and is certified mycoplasma free. 

This cell line is also available for purchase as a pair with the U266B1-Fluc-Puro (BCMA positive) cell line, for a discounted rate (link).