K562-Fluc-Puro/CyCD20-Neo
- Frozen / Standard (CL200) $ 3,000
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Species: Human
Cell Type: Chronic Myelogenous Leukemia
Transgenes: Firefly luciferase (Fluc; Luc2)
Cynomolgus macaque CD20 (CyCD20)
Selection Gene: Puromycin resistance (Puro)
Neomycin resistance (Neo)
Description:
The K562-Fluc-Puro/CyCD20-Puro cell line stably expresses firefly luciferase and cynomologus macaque CD20. The cells are designed for in vitro and in vivo studies of CD19. Parental K562-Fluc-Puro cells (Imanis #CL171) can be used as a negative control.
Imanis K562-Fluc-Puro cells (Imanis #CL171) cells were transduced with a cynomolgus macaque CD20-encoding lentivirus. A high CyCD20 expressing population was generated by selection using a methylcellulose based semi-solid medium.
This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.
Characterization:
Morphology
Phase cell photo taken at 200x magnification
Luciferase Expression
The indicated number of cells were placed in wells of a 96-well plate. After the addition of 15 mg/mL d-luciferin, bioluminescence was immediately read using a microplate reader.
CD20 Expression
K562-Fluc-Puro/CyCD120-Puro cells were stained with an anti-CyCD20 (blue) or isotype control (grey) antibody and analyzed by flow cytometry. (Note: the antibody used for analysis cross-reacts with both human and cynomolgus macaque CD20.)
Surface Receptor Profiling
K562-Fluc-Puro/CyCD20-Puro cells were stained with an anti-HuBCMA (A), anti-HuCD19 (B), anti-HuCD38 (C), or anti-HuGPRC5D (D) antibody and analyzed by flow cytometry.
