• 20 ug / Standard ((DNA1034-STAN)) $ 1,250

• Name of plasmid: pLV-SFFV-Luc2-P2A-Puro
• Description: pHR'-SIN-SFFV-Luc2-P2A-Puro plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
• Transgenes: Firefly luciferase (Luc2) and puromycin resistance (Puro)
• Linker: Self-cleaving peptide P2A
• Proposed Use: For generation of lentiviral vector stocks encoding dual transgenes firefly luciferase and puromycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: HeLaH1 cells were transduced with LV-Luc2-Puro and selected with puromycin. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.