pLV-SFFV-rhesusNIS
- 20 ug / Standard ((DNA1037-STAN)) $ 1,500
- Name of plasmid: pLV-SFFV-RhNIS
- Description: pHR'-SIN-SFFV-Rhesus NIS plasmid DNA
- Amount of DNA: 20 ug
- Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
- Transgene: Rhesus sodium iodide symporter (RhNIS)
- Proposed Use: For generation of lentiviral vector stocks encoding Rhesus NIS in a triple transfection protocol.
Plasmid Map:
Transgene Validation: Uptake assay of LV-RhNIS transduced cells in the presence or absence of KClO4, an inhibitor of NIS-mediated 125I uptake.