pLV-SFFV-rhesusNIS

• Name of plasmid: pLV-SFFV-RhNIS
• Description: pHR'-SIN-SFFV-Rhesus NIS plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
• Transgene: Rhesus sodium iodide symporter (RhNIS)
• Proposed Use: For generation of lentiviral vector stocks encoding Rhesus NIS in a triple transfection protocol.

Plasmid Map:

Transgene Validation: Uptake assay of LV-RhNIS transduced cells in the presence or absence of KClO₄, an inhibitor of NIS-mediated ¹²⁵I  uptake.