pLV-CAG-mouseNIS-P2A-Neo

• Name of plasmid: pLV-CAG-mouse NIS-P2A-Neo
• Description: pHR'-SIN-CAG-mNIS-P2A-Neo plasmid DNA
• Amount of DNA: 20 ug
• Promoter: Synthetic hybrid CAG promoter (CMV enhancer, chicken beta-Actin promoter and rabbit beta-Globin splice acceptor site) for constitutively high levels of gene expression in numerous cell types.
• Transgenes: Mouse sodium iodide symporter (mNIS) and neomycin resistance (Neo)
• Linker: Self-cleaving peptide P2A
• Proposed Use: For generation of lentiviral vector stocks encoding mouse NIS and neomycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: HeLaH1 cells were transduced with LV-CAG-mNIS-Neo and selected with G418. NIS mediated ¹²⁵I uptake was assayed in the presence or absence of KClO₄, a competitive inhibitor of iodine uptake.