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This is a polyclonal population derived from the EMT6 mammary carcinoma cell line (ATCC® CRL-2755™). Parental EMT6 cells were transduced with LV-Fluc-P2A-Puro (Imanis #LV012) encoding the firefly luciferase (Fluc) cDNA under the SFFV promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin. The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancers and promoters have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR¹.