This is a cell line derived from the human B cell precursor leukemia Nalm6 cell line (ATCC® CRL-3273TM). Parental Nalm6 cells were transduced with LV-Luc2-P2A-hNIS (Imanis #LV023) encoding the firefly luciferase (Fluc) cDNA under the spleen focus- forming virus (SFFV) promoter and linked to the human sodium iodide symporter (hNIS) cDNA via a P2A cleavage peptide. A high Fluc and hNIS expressing population was generated by two rounds of selection using a methylcellulose based semi-solid medium.