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Frozen / Standard
(CL125-STAN)
$ 2,100
This is a polyclonal population derived from the prostate PC3 cell line (ATCC® CRL-1435
TM). Parental PC3 cells were transduced with 1) LV-Fluc-P2A-Puro (Imanis #LV012) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide and 2) LV-eGFP-P2A-Neo (Imanis #LV067) encoding the enhanced green fluorescent protein
(eGFP) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide. High Fluc and eGFP expressing cells were selected using puromycin and G418, respectively.