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This is a polyclonal population derived from the prostate PC3 cell line (ATCC® CRL-1435^TM). Parental PC3 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide and 2) LV-iRFP-P2A-Puro (Imanis #LV032) encoding the near-infrared fluorescent protein (iRFP; ex/em = 690/713) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. High Fluc and iRFP expressing cells were selected using G418 and puromycin, respectively.