Anti-VSV antibody
- 0.25 ml / Standard (REA005-STAN) $ 495
Description: Rabbit polyclonal antibody against Vesicular Stomatitis Virus (VSV).
Antigen: Purified stock of VSV-hIFNβ virus (Indiana strain of VSV encoding human interferon beta, Imanis catalog # OV2011)
Species: Rabbit
Isotype: Sera
Tested Applications:
- Western blot (1:8,000 to 10,000)
- Immunofluorescence (1:1,500 to 1:3,000)
- Immunohistochemistry (1:5,000 to 10,000)
- ELISA
- Virus neutralization assay
- Flow cytometry analysis (1:10,000)
Note: Users are strongly advised to determine the optimal concentration of antibody to use for their specific applications.
Western Blot of VSV infected cells and VSV virions using Imanis anti-VSV Ab (catalog # REA005)
Lane 1: Total protein lysate of non-infected Vero cells (6 ug). Lane 2: Total protein of VSV infected Vero cells (6 ug). Lane 3: VSV viral stock at 8x104 TCID50 infectious units. Anti-VSV Ab (REA005) was used at 1:8000 dilution.
Lane 1: Total protein lysate of non-infected Vero cells (6 ug). Lane 2: Total protein of VSV infected Vero cells (6 ug). Lane 3: VSV viral stock at 8x104 TCID50 infectious units. Anti-VSV Ab (REA005) was used at 1:8000 dilution.
Immunofluorescence staining for VSV infection in tissue cryosections
Tumor xenografts treated with (A) VSV virotherapy or (B) saline were stained with the REA005 anti-VSV antibody (1:1500 dilution). Detection was with a red fluorescent secondary antibody.
