• 0.25 mL / Standard / Y ((OV4011-STAN)) $ 1,500

VV_(Li)-iTyr-GusA is a recombinant vaccinia virus (VV) encoding the following additional transcription units: 1) the tet-repressor (tetR) cDNA under transcriptional control of the VV synthetic early late (pSEL) promoter, 2) the tet-operator sequence tetO followed by mouse tyrosinase (mTYR) cDNA under transcriptional control of the VV synthetic late (pSL) promoter, and 3) the β-glucuronidase (GusA) cDNA under transcriptional control of p11 promoter. These additional transcription units are inserted into, and disrupt the function of, the viral F14.5L, J2R (thymidine kinase), and A56R (hemagglutinin) genes, respectively. Expression of tyrosinase is under the control of an inducible promoter-system, whereby addition of doxycycline induces tyrosinase expression (see diagram below). The purchased virus is ready for use in cell-killing assays or can be amplified to create additional virus stocks.