B16F10 (ATCC® CCL-6475™) is a murine melanoma cell line from a C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, and injecting them into fresh mice, a total of 10 times. The cells are adherent with an epithelial morphology. B16F10 cells are highly metastatic and will form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice.

Route of Implantation	Mice	Tumor/Metastases	References
Subcutaneous	C57BL/6	Subcutaneous tumor	Zhang et al. (2015) BMC Cancer 15: 234. Takeda et al. (2015) Blood 118: 464-472.
Tail vein	C57BL/6	Lung metastases	Zhang et al. (2015) BMC Cancer 15: 234. Takeda et al. (2015) Blood 118: 464-472. Fidler (1975) Cancer Res 35: 218-224. Burghoff et al. (2014) BMC Cancer 14: 898.
Subcutaneous	C57BL/6	Subcutaneous tumor, lung metastases	Fidler (1975) Cancer Res 35: 218-224.

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Our B16F10 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our B16F10 cells are available with a variety of different reporters, including the murine sodium iodide symporter (mNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter B16F10 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).  

For more information about a specific reporter and its uses, click on the photos below.