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Fluorescent Proteins

  • Lentiviruses
  • Plasmids
  • GFP
  • LV-eGFP starting at $ 225

  • LV-eGFP-P2A-human NIS starting at $ 225

  • LV-eGFP-PGK-Puro starting at $ 225

  • pLV-SFFV-eGFP starting at $ 660

  • pLV-SFFV-eGFP-P2A-human NIS starting at $ 1,150

  • pLV-SFFV-eGFP-PGK-Puro starting at $ 660

  • pLV-SFFV-human NIS-P2A-eGFP-T2A-Luc2 starting at $ 1,150

  • dsRed
  • pLV-SFFV-dsRed starting at $ 660

  • pLV-SFFV-dsRed-PGK-Puro starting at $ 660

  • Fluorescent Proteins representative image
    Fluorescent Proteins representative image

    Description

    Fluorescent proteins are optical reporters that, following excitation by an appropriate wavelength of light, emit light at a specific wavelength. Green fluorescent protein (GFP) is the most commonly used fluorescent reporter. Initially isolated from the jellyfish Aqueoria victoria, GFP has since been engineered to generate the brighter and more photostable enhanced GFP (eGFP), with an excitation wavelength of 488 nm and emission wavelength of 509 nm. Additional mutations of GFP have produced fluorescent proteins emitting different colors of light including yellow, cyan, red (DsRed), and ultraviolet. 

    The light emitted by fluorescent proteins can be used to image cells or viruses by conventional or intravital microscopy. Noninvasive optical imaging of fluorescent proteins in mice is hampered by tissue autofluorescence, which makes this type of imaging impractical. Microscopic analyses of fluorescent protein signal in post-mortem samples, however, is frequently used to verify the results of noninvasive imaging using different reporters.

    How to use fluorescent proteins for imaging

    Reagents: imaging fluorescent proteins requires no additional reagents, other than what may be needed to prepare post-mortem samples for microscopy. 

    Equipment: fluorescent proteins can be imaged using a conventional fluorescent microscope or an intravital microscope. It is important to confirm that the microscope to be used has an excitation laser and emission filter combinations that will work for the fluorescent protein of choice.

    • eGFP: excitation = 488 nm/emission = 509 nm
    • DsRed: excitation = 558 nm/emission = 583 nm

    Workflow:

    When to use fluorescent proteins 

    Due to tissue autofluorescence, fluorescent proteins are not recommended for noninvasive imaging studies. In mice and other small animals, fluorescent proteins can be used for intravital microscopy imaging to achieve cellular-level resolution. Due to the relative ease and low-cost of traditional microscopy, an ideal application for fluorescent proteins in in vivo imaging is post-mortem confirmation of noninvasive imaging results acquired with different reporters.

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    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

    Use Fewer Animals

    Our Reduction Campaign

    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

    What people say about Imanis

    Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

    – Dr. Raymond Hickey, Mayo Clinic

    Learning Resources