LL/2 Cells

Products

Description:

LL/2 or LLC1 (ATCC® CRL-1642™) is a murine Lewis lung carcinoma cell line from a C57BL mouse bearing a tumor formed by implantation of a primary Lewis lung carcinoma. The cells tend to be a mixed population of loosely attached and suspension cells, depending on the density, appearing either epithelial or rounded. LL/2 cells are highly tumorgenic and will form tumors and metastases post implantation into syngenic C57BL mice or immunocompromised mice.

Usage Information:

LL/2 cells are suitable for in vitro and in vivo experimentation. Both syngenic C57BL mice and immunocompromised mice can be used for in life studies, and will form tumors following implantation of the cells.

The following chart provides some examples of LL/2 cells used for tumor formation and studies.

Route of Implantation	Mice	Tumor/Metastases	References
Subcutaneous	C57BL6J	Subcutaneous tumor, limited lung metastases	Ito et al. (2013) PLoS One 8: e73931.
Subcutaneous	C57BL/6	Subcutaneous tumor	Soolev et al. (2009) Cancer Res 69: 2531-2539.
Tail vein	C57BL/6	Lung metastases	Takeda et al. (2015) Blood 118: 464-472. Ito et al. (2013) PLoS One 8: e73931. Mizumoto et al. (2013) BioMed Res International ID: 656319.

Route of Implantation

Mice

Tumor/Metastases

References

Subcutaneous

C57BL6J

Subcutaneous tumor, limited lung metastases

Ito et al. (2013) PLoS One 8: e73931.

Subcutaneous

C57BL/6

Subcutaneous tumor

Soolev et al. (2009) Cancer Res 69: 2531-2539.

Tail vein

C57BL/6

Lung metastases

Takeda et al. (2015) Blood 118: 464-472.

Ito et al. (2013) PLoS One 8: e73931.

Mizumoto et al. (2013) BioMed Res International ID: 656319.

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:

Our LL/2 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our LL/2 cells are available with a variety of different reporters, including the murine sodium iodide symporter (mNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter LL/2 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

For more information about a specific reporter and its uses, click on the photos below.

GFP