Mel624 is a human malignant melanoma cell line generated at Mayo Clinic. The cells are adherent and have an epithelial morphology. Mel624 cells will form tumors post-implantation into immunocompromised mice.

Route of Implantation	Mice	Tumor/Metastases	References
Subcutaneous	NSG	Subcutaneous tumor	Zheng et al. (2016) Oncotarget 7: 6448-6459
Tail vein	NSG	Nonspecific metastases	Zheng et al. (2016) Oncotarget 7: 6448-6459

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Our Mel624 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our Mel624 cells are available with the human sodium iodide symporter (hNIS) alone or in combination with enhanced green fluorescent protein (eGFP) to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998). 

For more information about a specific reporter and its uses, click on the photos below.