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Mel624 Cells

Mel624 Cells representative image
Mel624 Cells representative image

Description:

Mel624 is a human malignant melanoma cell line generated at Mayo Clinic. The cells are adherent and have an epithelial morphology. Mel624 cells will form tumors post-implantation into immunocompromised mice.

Usage Information:

Mel624 cells are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors following implantation of the cells.

The following chart provides from a study using Mel624 cells as a xenograft model.

Route of Implantation

Mice

Tumor/Metastases

References

Subcutaneous

NSG

Subcutaneous tumor

Zheng et al. (2016) Oncotarget 7: 6448-6459

Tail vein

NSG

Nonspecific metastases

Zheng et al. (2016) Oncotarget 7: 6448-6459

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines: 

Our Mel624 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our Mel624 cells are available with the human sodium iodide symporter (hNIS) alone or in combination with enhanced green fluorescent protein (eGFP) to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998). 

For more information about a specific reporter and its uses, click on the photos below.

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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