Measles Virus

Description

Type: (-) ssRNA enveloped paramyxovirus

Strain: Edmonston vaccine lineage (live attenuated)

Tropism: The Measles virus hemagglutinin (H) protein mediates binding to the following cellular receptors
  • CD46 receptor (most human tissue types)
  • SLAM receptor (immune-cells)
  • Nectin-4 receptor (throat epithelium)


    • Gene Map: (Arrows indicate transgene insertion sites)

     

    Proposed Uses

    Standard titer stocks are ready to use in cell killing assays or for generation of new viral stocks. High-titer purified stocks are ready for immediate use in in vivo studies.

    Protocol Tips

    Propagation: Inoculate producer cells (e.g. Vero cells) at an MOI of 0.02 for 2-3 hours. Collect the cells when 80-90% of the cell monolayer has formed syncytia. Lyse the cells by 2 freeze/thaw cycles in liquid nitrogen. Remove cell debris by centrifugation. Freeze and store aliquots at or below -70oC.

    Viral Titration: Titer virus by TCID50/mL endpoint dilution assay on Vero cells. Score CPE positive cells based on the presence of syncytia 4-5 days after inoculation.  

    Regulatory Information

    Biosafety Level: 1/2 (Check details on specific product pages)

    US shipping license required? No.