Measles-GFP
- 0.25 mL / Standard / N ((OV1006-STAN)) $ 1,250
Ready to use oncolytic MV-GFP virus
- Live attenuated Edmonston strain measles virus encoding green fluorescent protein (MV-GFP)
- Strain: Edmonston vaccine lineage
- Transgene: Green fluorescent protein (GFP), inserted upstream of the nucleocapsid (N) gene
- Biosafety Level: 1
- Reference: Myers et al. Hum Gene Ther. 2003 Nov, 14(16):1565-77.
- Titer: Standard (clarified supernatant, 3e5 to 1e6 TCID50/ml)
- Propagation method: Inoculate producer cell (e.g. Vero) at MOI of 0.02 for 2-3 hours. Harvest cells when 80-90% of cell monolayer has formed syncytia. Freeze/thaw 2 cycles and freeze aliquots.
- Proposed Use: Ready to use for generation of new viral stocks and in cell killing assays
- USDA permit required for shipping? No.
