Species: Human

Cell type: Melanoma

Transgenes: Human sodium iodide symporter (hNIS) with neomycin resistance (Neo) for selection with G418

Media: DMEM, 10% FBS, 1% Pen/Strep, 0.25mg/mL G418

Description: Mel624-hNIS-Neo is a monoclonal population of the human melanoma Mel624 cell line transduced with LV-hNIS-Neo (LV013) encoding the human sodium iodide symporter (hNIS) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the neomycin resistance gene via an IRES.

The lentiviral vector used is a self-inactivating (SIN) vector in which the viral enhancer and promoter has been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).

Recommended uses:

In vitro: This is a high hNIS expressing clone suitable for use as a positive control cell line in iodine uptake assays to validate NIS expression in your lentiviral transduced cells.

In vivo: This human melanoma cell line grows as subcutaneous xenografts in aythmic nude mice and its growth can be monitored noninvasively using high-resolution 3D SPECT or PET imaging.

References on NIS imaging:

1. Fruthwirth et al. A whole body dual modality radionuclide optical strategy for preclinical imaging of metastasis and heterogeneous treatment responses in different. microenvironments. J. Nucl. Med 2014. 55(4): 686-94.

2. Penheiter et al. The sodium iodide symporter (NIS) as an imaging reporter for gene, viral and cell-based therapies. Curr Gene Ther. 2012, 12(1):33-47.

Morphology: Low- and high-density cell morphology (200x)

NIS Function Assay (Iodine Uptake): Cells were incubated with ¹²⁵I for 1h in the presence or absence of KClO₄, an inhibitor of iodine uptake. Radioiodine concentrated within the cells was measured with a gamma counter.