High-titer purified LV-murine NIS-P2A-Neo lentivirus stocks

Promoter: Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in a variety of animal models.

Transgenes: Murine sodium iodide symporter (mNIS) linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide.

Envelope/Tropism: VSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.

Construct: This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).

Titer: Approximately 0.5-2e7 TU/mL (by qPCR titration of transduced HeLa H1 cells)

Proposed Use: In vitro transduction of primary cells and cell lines.

Publications that used associated LV-mNIS product:

1. Hickey et al. Noninvasive 3-dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene. Liver Transpl. 2015 Apr;21(4):442-53.

Vector Map:

Iodine (¹²⁵I) Uptake Assay of LV-mNIS-P2A-Neo transduced HeLa H1 cells: