LV-iRFP-P2A-Puro

High-titer purified LV-iRFP-P2A-Puro lentivirus stocks

Promoter: Spleen focus-forming virus (SFFV) promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, we find that SFFV works very well in animal models.

Transgenes: Near-infrared fluorescent protein (iRFP) linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. iRFP has excitation and emission of 690 and 713 nm, respectively. 

Envelope/Tropism: VSV.G pseudotyped HIV-1 based lentiviral vector capable of transducing a broad range of cell types and species.

Construct: This is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Thus, transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization of replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).

Titer: Approximately 0.5-2e7 TU/mL (by qPCR titration of transduced HeLa H1 cells)

Proposed Use: In vitro transduction of primary cells and cell lines.

Vector Map:

Flow Cytometry of LV-iRFP-P2A-Puro Transduced HeLa H1 Cells: