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HCT116 Cells

HCT116 Cells representative image
HCT116 Cells representative image

Description:

HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.

Usage Information:

In vitro, HCT116 cells grow with a doubling time of approximately 18 hours. They are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors and metastases following implantation of the cells.

The following chart provides some examples of HCT116 cells used as a xenograft model.

Route of Implantation

Mice

Tumor/Metastases

References

Intrasplenic

Nude

Liver metastases

Cui et al. (2014) Cancer Cell Interation 14: 47.

Tail-vein

Nude

Lung metastases

Gao et al. (2015) Oncogene 34: 4142-4152.

Tail-vein

SCID

Lung metastases

Zhou et al. (2016) BMC Cancer 16: 55.

Subcutaneous

Nude

Subcutaneous tumor

Gao et al. (2015) Oncogene 34: 4142-4152.

Intracaecum

Nude

Caecal tumors, lung, liver, and lymph node metastases

Yang et al. (1997) Anticancer Res 17: 3463-3468.

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines: 

Our HCT116 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our HCT116 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter HCT116 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).  

For more information about a specific reporter and its uses, click on the photos below.

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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