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Near-infrared Fluorescent Protein

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  • LV-iRFP-P2A-human NIS starting at $ 225

  • LV-iRFP-P2A-Neo starting at $ 225

  • LV-iRFP-P2A-Puro starting at $ 225

  • pLV-SFFV-iRFP-P2A-human NIS starting at $ 1,150

  • pLV-SFFV-iRFP-P2A-Neo starting at $ 660

  • pLV-SFFV-iRFP-P2A-Puro starting at $ 660

  • Near-infrared Fluorescent Protein representative image
    Near-infrared Fluorescent Protein representative image

    Description 

    Near infrared-fluorescent protein (iRFP) is an engineered version of the bacteriophytochrome RpBhP2 from the bacteria Rhodopseudomonas palustris. Like other fluorescent proteins, it emits a distinct wavelength of light (peak at 713 nm) after excitation at an appropriate wavelength (peak at 690 nm).

    Due to its red-shifted excitation/emission spectrum, iRFP has much lower tissue background than the other fluorescent proteins. Thus, the light emitted by iRFP can be used for noninvasive optical imaging as well as conventional microscopy.

    How to use iRFP for imaging

    Reagents: iRFP imaging does not require any additional reagents.

    Equipment: An optical imager with cooled CCD cameras can be used to detect the light produced by iRFP. The imager must be equipped with an appropriate laser and filter combination (e.g. 675/720 nm) to provide the excitation light and filter the iRFP emitted light. The acquired fluorescence image can be overlayed with a photograph taken by the optical imager to show the relative location of the fluorescent signal.

    When to use iRFP

    Noninvasive fluorescence imaging with iRFP can be used to noninvasively track the biodistribution of cells or viruses in mice and other small animals. Due to its relative ease of use, iRFP is a good reporter option for short-term pre-clinical studies where the signal is relatively close (1-2 cm) to the surface of the animal and high spatial resolution and sensitivity are not required.

    The iRFP signal is readily attenuated by tissues and should not be used for deep-tissue imaging. Also, as with bioluminescent images, fluorescence imaging produces surface-weighted images with relatively low spatial resolution. As a foreign protein, iRFP is immunogenic and should not be used in immune competent animals for longitudinal studies.

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    Use Fewer Animals

    Our Reduction Campaign

    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

    What people say about Imanis

    Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

    – Dr. Raymond Hickey, Mayo Clinic

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