pLV-SFFV-iRFP-P2A-Puro

• Name of plasmid: pLV-SFFV-iRFP-P2A-Puro
• Description: pHR' SIN-SFFV-iRFP-P2A-Puro plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
• Transgenes: Near-infrared fluorescent protein (iRFP; excitation: 690nm, emission: 713nm) and puromycin resistance (Puro)
• Linker: Self-cleavage peptide P2A
• Proposed Use: For generation of lentiviral vector stocks encoding the near-infrared fluorescent protein and puromycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: LV-iRFP-Puro transduced (red) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.