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Vaccinia Virus

Vaccinia Virus representative image
Vaccinia Virus representative image

Description

Type: Linear dsDNA enveloped poxvirus

Tropism: Vaccinia virus enters cells via macropinocytosis and unknown cellular receptors giving the virus a broad tropism (most mammalian cell types)


Gene Map of VV(Li)-RucGFP-hNIS-tRFP:

 

Proposed Uses

Standard titer stocks are ready to use in cell killing assays or for generation of new viral stocks. High-titer purified stocks are ready for immediate use in in vivo studies.

Protocol Tips

Propagation: Inoculate producer cells (e.g. Vero) at an MOI of 0.1 for 2-3 hours. After 48 hours harvest culture supernatant and cells and freeze-thaw or sonicate three times. Clarify the supernatant by low-speed centrifugation and purify through a  sucrose cushion.  Resuspend the viral pellet in 1mM TrisCl, pH 9.

Viral Titration: Titer virus by TCID50/mL endpoint dilution assay. Score CPE positive cells based on the presence of dead cells 3-5 days after inoculation. Alternatively, titer viruses by plaque assay and crystal violet staining. Vero cells can be used for TCID50 titrations.

Regulatory Information

Biosafety Level: 2

USDA transport permit required? Yes, you must have a USDA transport permit to ship Vaccinia virus. Please visit the USDA website to apply.

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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