Vaccinia vTF7-3
- 0.25 mL / Standard / Y (REA006-STAN) $ 495
Ready to use vaccinia TF7-3 virus
- Description: Replication competent vaccinia virus encoding the bacteriophage T7 RNA polymerase as a transgene.
- Strain: Vaccinia (Western Reserve WR strain)
- Transgene: Bacteriophage T7 RNA polymerase
- Biosafety Level: 2
- Reference: Fuerst et al., PNAS. 1986, 83(21): 8122-8126.
- Titer: Standard (clarified supernatant, 3e5 to 1e6 TCID50/ml).
- Propagation method: Inoculate producer cell (e.g. Vero or BHK-21) at MOI of 0.02 for 2-3 hours. Harvest supernatant when 80-90% of cell monolayer is showing cytopathic effects (CPE).
- Proposed Use: Ready to use viral stock for rescue of recombinant viruses from plasmid DNA encoding viral genes driven by a T7 RNA polymerase promoter.
