• Frozen / Standard (CL126-STAN) $ 2,500

This is a monoclonal population derived from the mammary carcinoma 4T1 cell line (ATCC® CRL-2539^TM). Parental 4T1 cells were transduced with LV-Luc2-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the neomycin resistance gene (Neo) cDNA via a P2A cleavage peptide. A monoclonal population was isolated following selection with G418 and two rounds of selection in a methylcellulose-based semi-solid medium. The lentiviral vector is a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR¹.